Huazhong University of Science and Technology, Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, 1037 Luoyu Road, Wuhan 430074, China.
J Biomed Opt. 2012 Jun;17(6):061207. doi: 10.1117/1.JBO.17.6.061207.
Because cerebral hypoperfusion brings damage to the brain, prevention of cerebrovascular diseases correlative to hypoperfusion by studying animal models makes great sense. Since complicated cerebrovascular adaptive changes in hypoperfusion could not be revealed only by cerebral blood flow (CBF) velocity imaging, we performed multi-parameter imaging by combining laser speckle imaging and functional photoacoustic microscopy. The changes in CBF, hemoglobin oxygen saturation (SO(2)), and total hemoglobin concentration (HbT) in single blood vessels of ipsilateral cortex were observed during transient cerebral hypoperfusion by ligating the unilateral common carotid artery in rats. CBF, SO(2), and HbT, respectively, decreased to 37 ± 3%, 71 ± 7.5%, and 92 ± 1.3% of baseline in 6 s immediately after occlusion, and then recovered to 77 ± 4.8%, 84 ± 8%, and 96 ± 2% of baseline in 60 s. These parameters presented the decrease with different degree and the following recovery over time after ligation, the recovery of SO(2) lagged behind those of CBF and HbT, which had the similar response. The results demonstrated that complete monitoring of both cerebral hemodynamic response and oxygen metabolic changes occurred at the earliest period of cerebral hypoperfusion was possible by using the two image modalities with high temporal and spatial resolution.
由于脑灌注不足会对大脑造成损害，因此通过研究动物模型来预防与灌注不足相关的脑血管疾病具有重要意义。由于仅通过脑血流 (CBF) 速度成像无法揭示灌注不足时复杂的脑血管适应性变化，我们通过结合激光散斑成像和功能光声显微镜进行了多参数成像。通过在大鼠单侧颈总动脉结扎时观察到短暂性脑灌注不足期间同侧皮质单个血管中的 CBF、血红蛋白氧饱和度 (SO(2)) 和总血红蛋白浓度 (HbT) 的变化。CBF、SO(2) 和 HbT 分别在闭塞后 6 秒内降至基线的 37 ± 3%、71 ± 7.5% 和 92 ± 1.3%，然后在 60 秒内恢复至基线的 77 ± 4.8%、84 ± 8% 和 96 ± 2%。这些参数在结扎后呈现不同程度的下降和随后的恢复，SO(2) 的恢复滞后于 CBF 和 HbT 的恢复，它们具有相似的反应。结果表明，使用具有高时空分辨率的两种成像模式可以最早监测到脑灌注不足期间的脑血流动力学反应和氧代谢变化。
