Ecole polytechnique, LPICM, CNRS, Palaiseau 91128, France.
J Biomed Opt. 2012 Jun;17(6):066009. doi: 10.1117/1.JBO.17.6.066009.
Healthy human colon samples were analyzed ex vivo with a multispectral imaging Mueller polarimeter operating from 500 to 700 nm in a backscattering configuration with diffuse light illumination impinging on the innermost tissue layer, the mucosa. The intensity and polarimetric responses were taken on whole tissues first and after progressive exfoliation of the outer layers afterwards. Moreover, these measurements were carried out with two different substrates (one bright and the other dark) successively placed beneath each sample, allowing a reasonably accurate evaluation of the contributions to the overall backscattered light by the various layers. For the shorter investigated wavelengths (500 to 550 nm) the major contribution comes from mucosa and submucosa, while for the longer wavelengths (650 to 700 nm) muscular tissue and fat also contribute significantly. The depolarization has also been studied and is found to be stronger in the red part of the spectrum, mainly due to the highly depolarizing power of the muscular and fat layers.
健康的人类结肠样本在离体条件下使用多光谱成像 Mueller 偏振仪进行分析，该偏振仪在背向散射配置下工作，漫散光照射到最内层组织层，即黏膜。首先对整个组织进行强度和偏振响应测量，然后再对随后逐渐剥落的外层进行测量。此外，这些测量是在两个不同的基底（一个亮基底和一个暗基底）上进行的，这些基底分别放置在每个样本下方，从而可以对各个层对总背散射光的贡献进行合理准确的评估。对于较短的研究波长（500 至 550nm），主要贡献来自黏膜和黏膜下层，而对于较长的波长（650 至 700nm），肌肉组织和脂肪也有很大的贡献。还研究了退偏度，发现它在光谱的红色部分更强，主要是由于肌肉和脂肪层具有很强的退偏能力。
