Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USA.
Chem Commun (Camb). 2012 Aug 7;48(61):7580-2. doi: 10.1039/c2cc33280a. Epub 2012 Jun 26.
A methylene-blue (MB)-labeled molecular beacon junction probe allows for a signal-on electrochemical detection of nucleic acids via target recycling using endonucleases. Electron transfer is reduced when the MB is intercalated in the stem of the molecular beacon, but then electron transfer from MB to a gold electrode is enhanced upon cleavage of the junction probe due to increased probability of MB approaching the electrode when attached to the more flexible ssDNA.
一种亚甲蓝（MB）标记的分子信标连接探针可通过使用内切酶进行目标循环利用实现信号开启的电化学核酸检测。当 MB 嵌入分子信标的茎部时，电子转移会减少，但由于连接探针的切割增加了 MB 接近电极的可能性，当 MB 连接到更灵活的 ssDNA 时，电子从 MB 转移到金电极的概率会增强。
