Department of Animal Science, University college of Agriculture and Natural Resources, University of Tehran, Karaj, Islamic Republic of Iran.
Gene. 2012 Sep 1;505(2):379-83. doi: 10.1016/j.gene.2012.06.009. Epub 2012 Jun 23.
Growth hormone secretagogue receptor (GHSR), a G protein-coupled receptor that binds ghrelin, plays an important role in the central regulation of pituitary growth hormone secretion, food intake, and energy homeostasis. Ghrelin receptor (GHSR) modulates many physiological effects and therefore is a candidate gene for sheep production performance. Polymorphism of the GHSR gene was detected by PCR-SSCP and DNA sequencing methods in 463 individuals. Two different structures in protein and nine single nucleotide polymorphisms (SNPs) were identified. The evaluation of the associations between these SSCP patterns with carcass traits suggests a positive effect of genotype TT and B structure on carcass weight, and body length (P<0.05). In addition, the animal with TC had greater abdominal fat than those with TT and CC (P<0.05) while CC genotype contributed to low blood cholesterol (P=0.04). The results confirm the hints suggesting that GHSR is a preferential target for further investigation on mutations that influence carcass trait variations.
生长激素促分泌受体（GHSR）是一种与 ghrelin 结合的 G 蛋白偶联受体，在垂体生长激素分泌、摄食和能量平衡的中枢调节中发挥重要作用。Ghrelin 受体（GHSR）调节许多生理效应，因此是绵羊生产性能的候选基因。使用 PCR-SSCP 和 DNA 测序方法在 463 个人中检测到 GHSR 基因的多态性。鉴定出两种不同的蛋白质结构和九个单核苷酸多态性（SNP）。这些 SSCP 模式与胴体性状之间的关联评估表明 TT 和 B 结构基因型对胴体重和体长有积极影响（P<0.05）。此外，TC 基因型的动物比 TT 和 CC 基因型的动物具有更大的腹部脂肪（P<0.05），而 CC 基因型有助于降低血液胆固醇（P=0.04）。结果证实了这一暗示，即 GHSR 是进一步研究影响胴体性状变化的突变的首选目标。
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