Research Centre for Plant RNA Signalling, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China.
Sci Rep. 2012;2:467. doi: 10.1038/srep00467. Epub 2012 Jun 22.
In plants, non-cell autonomous RNA silencing spreads between cells and over long distances. Recent work has revealed insight on the genetic and molecular components essential for cell-to-cell movement of RNA silencing in Arabidopsis. Using a local RNA silencing assay, we report on a distinct mechanism that may govern the short-range (6-10 cell) trafficking of virus-induced RNA silencing from epidermal to neighbouring palisade and spongy parenchyma cells in Nicotiana benthamiana. This process involves a previously unrecognised function of the RNA-dependent RNA polymerase 6 (RDR6) gene. Our data suggest that plants may have evolved distinct genetic controls in intercellular RNA silencing among different types of cells.
在植物中，非细胞自主的 RNA 沉默在细胞之间以及长距离内传播。最近的工作揭示了 RNA 沉默在拟南芥中进行细胞间运动所必需的遗传和分子成分的深入见解。使用局部 RNA 沉默测定法，我们报告了一种可能控制病毒诱导的 RNA 沉默从表皮到相邻栅栏薄壁组织细胞和海绵状薄壁组织细胞的短程（6-10 个细胞）运输的独特机制在本生烟中。这个过程涉及 RNA 依赖性 RNA 聚合酶 6 (RDR6) 基因的一个以前未被识别的功能。我们的数据表明，植物可能在不同类型的细胞之间的细胞间 RNA 沉默中进化出不同的遗传控制。
