Ledbetter Eric C, da Silva Erotides C, Kim Sung G, Dubovi Edward J, Schwark Wayne S
Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
Am J Vet Res. 2012 Jul;73(7):1079-84. doi: 10.2460/ajvr.73.7.1079.
To determine the frequency of spontaneous canine herpesvirus-1 (CHV-1) reactivation and ocular viral shedding in latently infected dogs and the effect of topical ocular administration of cyclosporine.
8 mature Beagles with experimentally induced latent CHV-1 infection.
Following induction of primary ocular CHV-1 infection, the presence of reactivatable CHV-1 latency was confirmed by systemically administering prednisolone to the dogs. Dogs were then monitored for 36 weeks via clinical examination and conjunctival sample CHV-1 PCR assay performed at 4-day intervals and CHV-1 virus neutralization antibody assay performed at 2-week intervals. During weeks 16 to 32, dogs were administered 0.2% cyclosporine ointment in both eyes twice daily and blood cyclosporine concentrations were monitored. During weeks 33 to 36, the presence of reactivatable CHV-1 latency was reconfirmed via systemic administration of prednisolone.
Reactivation of latent CHV-1 was not detected via clinical examination or viral shedding during the initial 32 weeks, including before and during topical ocular administration of cyclosporine, and there were no significant differences in CHV-1 virus neutralization titer increases between the study periods. Blood cyclosporine concentrations were less than assay detection limits in all dogs on the sampling days. Systemic administration of corticosteroids repeatedly resulted in ocular disease and viral shedding.
Spontaneous CHV-1 reactivation did not occur frequently in latently infected mature dogs, and this was not altered by topical ocular administration of cyclosporine. This characteristic may be a factor contributing to the lower frequency of recurrent herpetic ocular disease in dogs relative to other host species and their associated alphaherpesviruses.
确定潜伏感染犬自发性犬疱疹病毒1型（CHV-1）激活及眼部病毒排出的频率，以及局部眼部应用环孢素的效果。
8只经实验诱导产生潜伏性CHV-1感染的成年比格犬。
在原发性眼部CHV-1感染诱导后，通过给犬全身应用泼尼松龙来确认可激活的CHV-1潜伏状态的存在。然后通过临床检查对犬进行36周的监测，每隔4天进行结膜样本CHV-1聚合酶链反应（PCR）检测，每隔2周进行CHV-1病毒中和抗体检测。在第16至32周期间，给犬双眼每日两次应用0.2%环孢素眼膏，并监测血液中环孢素的浓度。在第33至36周期间，通过全身应用泼尼松龙再次确认可激活的CHV-1潜伏状态的存在。
在最初32周内，包括局部眼部应用环孢素之前和期间，通过临床检查或病毒排出均未检测到潜伏性CHV-1激活，且研究期间CHV-1病毒中和滴度增加无显著差异。在采样日，所有犬的血液中环孢素浓度均低于检测限。全身应用皮质类固醇反复导致眼部疾病和病毒排出。
潜伏感染的成年犬中自发性CHV-1激活不常见，且局部眼部应用环孢素未改变这一情况。这一特征可能是犬相对于其他宿主物种及其相关α疱疹病毒而言，疱疹性眼病复发频率较低的一个因素。
