Zhang Ying, Chen Rui, Zhang Wei, Li Juan, Chen Xuan, Zhao Li
The First Hospital of Lanzhou University, Lanzhou, Gansu Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2012 Jun;20(3):531-5.
This study was purposed to investigate the expression level of oncogene c-myc and c-myb in leukemic cells and leukemic bone marrow stromal cells (BMSC) and the correlation with each other. The expression levels of c-myc and c-myb in those cells were examined semi-quantitatively by reverse transcription polymerase chain reaction (RT-PCR). Flow cytometry analysis was used to detect the membrane surface antigens of leukemic cells and BMSC. The karyotype was analyzed by G-banding techniques. The results showed that (1) c-myc and c-myb gene were expressed in the normal control group, the leukemic cells and BMSC of patients group. The mean expression levels of c-myb mRNA and c-myc mRNA in abnormal chromosomal leukemic cells were 1.03 ± 0.48 and 1.15 ± 0.38 respectively, which were significantly higher than those in control group (P < 0.05). In the abnormal karyotype stromal cells, the mean expression level of c-myb mRNA and c-myc mRNA were 2.08 ± 0.82 and 1.46 ± 0.29 respectively (P < 0.05). (2) The expression level of c-myc and c-myb mRNA were closely associated with patients' platelet counts (P < 0.05). (3) The expression of c-myc mRNA linearly correlated with the expression of c-myb mRNA in different prognostic groups. (4) In acute leukemic cells and BMSC, c-myc expression positively correlated with c-myb expression. (5) The expression level of c-myc in leukemic cells correlated with the expression levels of c-myc and c-myb in BMSC, respectively. It is concluded that the reduction of c-myc or c-myb expression levels may be a therapeutic regimen for leukemia.
本研究旨在探讨癌基因c-myc和c-myb在白血病细胞及白血病骨髓基质细胞（BMSC）中的表达水平及其相互关系。采用逆转录聚合酶链反应（RT-PCR）半定量检测这些细胞中c-myc和c-myb的表达水平。运用流式细胞术分析检测白血病细胞和BMSC的膜表面抗原。采用G显带技术分析核型。结果显示：（1）c-myc和c-myb基因在正常对照组、患者组的白血病细胞及BMSC中均有表达。染色体异常的白血病细胞中c-myb mRNA和c-myc mRNA的平均表达水平分别为1.03±0.48和1.15±0.38，显著高于对照组（P<0.05）。在核型异常的基质细胞中，c-myb mRNA和c-myc mRNA的平均表达水平分别为2.08±0.82和1.46±0.29（P<0.05）。（2）c-myc和c-myb mRNA的表达水平与患者血小板计数密切相关（P<0.05）。（3）在不同预后组中，c-myc mRNA的表达与c-myb mRNA的表达呈线性相关。（4）在急性白血病细胞和BMSC中，c-myc表达与c-myb表达呈正相关。（5）白血病细胞中c-myc的表达水平分别与BMSC中c-myc和c-myb的表达水平相关。结论：降低c-myc或c-myb的表达水平可能是白血病的一种治疗方案。
