School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, China.
J Sep Sci. 2012 Jun;35(12):1432-8. doi: 10.1002/jssc.201200096.
The combination of molecularly imprinted polymer with high performance liquid chromatography has been developed to determine cyromazine and its metabolic melamine in some samples. However, the potential risk of template leakage used in molecularly imprinted polymer is a major disadvantage. To solve this problem, 2-(4,6-diamino-1,3,5-triazin-2-ylamino) ethanethiol disulfide, a molecule that shares the similar imprinting sites with cyromazine and melamine, was selected as pseudo template to prepare molecularly imprinted polymer. Methacrylic acid, ethylene glycol dimethyl acrylate and toluene were selected as functional monomer, crosslinker and porogen, respectively. The molecular recognition property and binding capability of cyromazine and melamine were evaluated by adsorption test and Scatchard analysis. The results showed that the molecularly imprinted polymer based on pseudo template had more excellent affinity and selectivity for cyromazine and melamine. The resulting molecularly imprinted polymer was used as a solid-phase extraction material to enrich cyromazine and melamine in egg and milk samples for high performance liquid chromatography analysis. The solid-phase extraction process was carefully optimized. It was found that when different concentration of cyromazine and melamine standards were spiked into samples, satisfactory recovery rate of cyromazine and melamine were obtained as 85.6-98.8% with relative standard deviation <5.5%.
采用分子印迹聚合物与高效液相色谱相结合的方法来检测一些样品中的环丙氨嗪及其代谢产物三聚氰胺。然而，分子印迹聚合物中模板分子的潜在泄漏风险是其主要的缺点。为了解决这个问题，选择了 2-（4,6-二氨基-1,3,5-三嗪-2-基氨基）乙硫醇二硫化物作为假模板来制备分子印迹聚合物。甲基丙烯酸、乙二醇二甲基丙烯酸酯和甲苯分别被选为功能单体、交联剂和致孔剂。通过吸附试验和 Scatchard 分析评价了环丙氨嗪和三聚氰胺的分子识别性能和结合能力。结果表明，基于假模板的分子印迹聚合物对环丙氨嗪和三聚氰胺具有更好的亲和力和选择性。所得的分子印迹聚合物被用作固相萃取材料，用于高效液相色谱分析鸡蛋和牛奶样品中环丙氨嗪和三聚氰胺的富集。固相萃取过程被仔细优化。结果发现，当不同浓度的环丙氨嗪和三聚氰胺标准品加入到样品中时，可获得 85.6-98.8%的令人满意的回收率，相对标准偏差<5.5%。
