Department of Biology, University of Payamenoor, Isfahan, Iran.
Pharmacol Biochem Behav. 2012 Jul;102(1):77-81. doi: 10.1016/j.pbb.2012.03.009. Epub 2012 Mar 27.
Drug addiction is a chronic disorder characterized by compulsive drug-seeking behavior despite severe negative consequences. Most abused drugs increase dopamine release in the ventral tegmental area (VTA) and in the nucleus accumbens (NA). The medial prefrontal cortex (mPFC), a part of the mesocorticolimbic dopaminergic system, receives dopaminergic projections from VTA; and in turn, sends glutamatergic projections to both VTA and NA. The present study was designed to further investigate the involvement of the mPFC in the release of dopamine in the VTA by using in vivo microdialysis and high performance liquid chromatography with electrochemical detection (HPLC–ECD). Electrical lesion of the mPFC decreased the level of dopamine in the VTA to approximately 26.8% of basal level. Acute morphine (40 mg/kg i.p.) treatment increased the level of dopamine in the VTA, while the lesion of mPFC immediately before morphine administration attenuated the effects of acute morphine on the level of dopamine. These results suggest that the mPFC modulates dopamine release into the VTA.
药物成瘾是一种慢性疾病，其特征是尽管存在严重的负面后果，但仍存在强迫性药物寻求行为。大多数滥用药物会增加腹侧被盖区 (VTA) 和伏隔核 (NA) 中的多巴胺释放。内侧前额叶皮层 (mPFC) 是中边缘多巴胺能系统的一部分，接收来自 VTA 的多巴胺能投射；反过来，它向 VTA 和 NA 发送谷氨酸能投射。本研究旨在通过使用体内微透析和高效液相色谱电化学检测 (HPLC-ECD) 进一步研究 mPFC 在 VTA 中多巴胺释放中的作用。mPFC 的电损伤将 VTA 中的多巴胺水平降低到基础水平的约 26.8%。急性吗啡 (40mg/kg i.p.) 治疗增加了 VTA 中的多巴胺水平，而在给予吗啡之前立即损伤 mPFC 则减弱了急性吗啡对多巴胺水平的影响。这些结果表明，mPFC 调节多巴胺向 VTA 的释放。
