内皮素对神经母细胞瘤NG108 - 15和NCB - 20细胞胞浆游离钙浓度的影响。

Effect of endothelins on cytosolic free calcium concentration in neuroblastoma NG108-15 and NCB-20 cells.

作者信息

Yue T L, Gleason M M, Lysko P G, Feuerstein G

机构信息

Department of Pharmacology, Smith Kline Beecham, King of Prussia, PA 19406-0939.

出版信息

Neuropeptides. 1990 Sep;17(1):7-12. doi: 10.1016/0143-4179(90)90134-k.

DOI:10.1016/0143-4179(90)90134-k

PMID:2274119

Abstract

The effects of endothelin(ET)-1, ET-2, ET-3 and Big ET on intracellular level of Ca2+ ([Ca2+]i) were studied in neuroblastoma NG108-15 and NCB-20 cells. All ETs, except Big ET, induced an increase in [CA2+]i in NG108-15 cells in a dose-dependent manner, with EC50: 6.7, 11.2 and 71 nM, respectively. However, none of the ET increased [Ca2+]i in NCB-20 cells. Calcium channel blockers diltiazem or nicardipine had no effect on ET-induced increase in [CA2+]i, but extracellular Ca2(+)-depletion significantly reduced the response of NG108-15 cells to ETs. NG108-15 cells exhibited a homologous desensitization to sequential addition of ETs, but no heterologous desensitization among ET, bradykinin and PAF was observed. These data suggest that ET-induced receptor activation results in increased intracellular Ca2+ via a non voltage calcium channel mechanism and intracellular Ca2+ release.

摘要

在神经母细胞瘤NG108 - 15和NCB - 20细胞中研究了内皮素（ET）-1、ET - 2、ET - 3和大内皮素对细胞内钙离子水平（[Ca2+]i）的影响。除大内皮素外，所有内皮素均以剂量依赖方式诱导NG108 - 15细胞中[Ca2+]i升高，其半数有效浓度（EC50）分别为6.7、11.2和71 nM。然而，没有一种内皮素能使NCB - 20细胞中的[Ca2+]i升高。钙通道阻滞剂地尔硫卓或尼卡地平对内皮素诱导的[Ca2+]i升高无影响，但细胞外钙离子耗竭显著降低了NG108 - 15细胞对内皮素的反应。NG108 - 15细胞对内皮素的连续添加表现出同源脱敏，但在内皮素、缓激肽和血小板活化因子之间未观察到异源脱敏。这些数据表明，内皮素诱导的受体激活通过非电压钙通道机制和细胞内钙释放导致细胞内钙离子增加。