胆碱能去神经支配后小鼠海马中乙酰胆碱酯酶的膜结合型和可溶性分子形式的变化。

Changes in membrane-bound and soluble molecular forms of acetylcholinesterase in mouse hippocampus after cholinergic denervation.

作者信息

Schegg K M, Gillespie R P, Prym U, Peacock J H

机构信息

Department of Medicine, University of Nevada School of Medicine, Reno.

出版信息

Neurosci Lett. 1990 Oct 16;118(2):197-200. doi: 10.1016/0304-3940(90)90625-j.

DOI:10.1016/0304-3940(90)90625-j

PMID:2274269

Abstract

In order to observe acetylcholinesterase (AChE) and its distribution into soluble and membrane-bound molecular forms in cholinergically denervated hippocampus, AChE was analyzed in the hippocampus of adult mice with and without bilateral fornix lesions made by stereotactically positioned knife cuts. Homogenates from lesioned mice contained an average of 3% of choline acetyltransferase-specific activity, 12% of AChE-specific activity and 98% of protein compared to homogenates from controls. After lesioning, the relative proportion of membrane-bound G4 decreased from 77% to 56% of total AChE while soluble G4, membrane-bound G1-G2, and soluble G1-G2 each increased in relative abundance.

摘要

为了观察胆碱能去神经支配海马中乙酰胆碱酯酶（AChE）及其在可溶性和膜结合分子形式中的分布，对成年小鼠进行立体定位刀切双侧穹窿损伤，分别分析损伤组和未损伤组小鼠海马中的AChE。与对照组匀浆相比，损伤组小鼠匀浆中胆碱乙酰转移酶特异性活性平均为3%，AChE特异性活性为12%，蛋白质含量为98%。损伤后，膜结合G4在总AChE中的相对比例从77%降至56%，而可溶性G4、膜结合G1-G2和可溶性G1-G2的相对丰度均增加。