Suhonen J, Koistinaho J, Hervonen A
Department of Public Health, University of Tampere, Finland.
Neurosci Lett. 1990 Oct 16;118(2):215-8. doi: 10.1016/0304-3940(90)90630-r.
Adrenal medullary tissue was autotransplanted to the superior cervical ganglion (SCG) of aged (26 months old) and young adult (3 months old) rats. Four and 20 weeks after operation, the viability of the transplants was evaluated using the formaldehyde-induced fluorescence (FIF) technique and tyrosine hydroxylase (TH) immunocytochemistry. Four weeks postgrafting, the transplant consisted of a densely-packed group of intensively fluorescent chromaffin cells in both age groups. The cells showed strong TH immunoreactivity and some of them were elongated, but only a few displayed short processes. At 20 weeks, most of the cells were spindle shaped and sent out fluorescent processes and a few of them were transformed toward ganglion-like cells. The results suggest that both young and old adrenal chromaffin cells are able to survive, produce neuronal processes and transform toward a neuronal phenotype in the rat superior cervical ganglion.
将肾上腺髓质组织自体移植到老年（26个月大）和年轻成年（3个月大）大鼠的颈上神经节（SCG）。术后4周和20周，使用甲醛诱导荧光（FIF）技术和酪氨酸羟化酶（TH）免疫细胞化学评估移植组织的活力。移植后4周，两个年龄组的移植组织均由密集排列的强荧光嗜铬细胞组成。这些细胞显示出强烈的TH免疫反应性，其中一些细胞呈细长形，但只有少数细胞显示出短突起。在20周时，大多数细胞呈纺锤形并发出荧光突起，其中一些细胞向神经节样细胞转化。结果表明，年轻和老年肾上腺嗜铬细胞都能够在大鼠颈上神经节中存活、产生神经突起并向神经元表型转化。
