School of Chemical Engineering and Technology, Harbin Institute of Technology, No. 92, West Da-Zhi Street, Harbin, 150001, China,
Adv Biochem Eng Biotechnol. 2013;131:121-52. doi: 10.1007/10_2012_135.
The lipid bilayer is one of the most important self-assembled structures in nature. In addition to compartmentalizing the cell, the lipid bilayer maintains many physical and biological characteristics of cell membranes, including lateral fluidity. It provides a wealth of opportunities for the study of membrane properties. Recently there has been an increasing interest in lipid bilayer arrays fabrication and their applications. In this review, the leading methods for creating lipid bilayer arrays are categorized as mechanical methods, pre-patterning substrate, direct UV patterning, direct blotting or stamping, polymer lift off technique, robotic micro spotting, and microfluidics. The applications of bilayer arrays for cell adhesion and activation, lipid bilayer based 2D electrophoresis, and high-throughput binding assays are also presented.
脂质双层是自然界中最重要的自组装结构之一。除了分隔细胞外，脂质双层还维持着细胞膜的许多物理和生物学特性，包括侧向流动性。它为研究膜特性提供了丰富的机会。最近，人们对脂质双层阵列的制造及其应用越来越感兴趣。在这篇综述中，将创建脂质双层阵列的主要方法分为机械方法、预图案化基底、直接紫外图案化、直接印迹或冲压、聚合物剥离技术、机器人微点样和微流控。还介绍了双层阵列在细胞黏附和激活、基于脂质双层的二维电泳和高通量结合分析中的应用。
