豚鼠白细胞介素-1β和单核细胞趋化蛋白-1的原核表达及体外功能分析
Prokaryotic expression and in vitro functional analysis of IL-1β and MCP-1 from guinea pig.
机构信息
Department of Microbial and Molecular Pathogenesis, Texas A&M Health Science Center, College Station, TX, USA.
出版信息
Mol Biotechnol. 2013 Jun;54(2):312-9. doi: 10.1007/s12033-012-9574-z.
The Guinea pig (Cavia porcellus) is an excellent animal model for studying human tuberculosis (TB) and also for a number of other infectious and non-infectious diseases. One of the major roadblocks in effective utilization of this animal model is the lack of readily available immunological reagents. In order to address this issue, guinea pig interleukin 1 beta (IL-1β) and monocyte chemoattractant protein-1 (MCP-1) were efficiently cloned and expressed in a prokaryotic expression vector, and the expressed proteins in soluble form from both the genes were confirmed by N-terminal sequencing. The biological activity of recombinant guinea pig IL-1β was demonstrated by its ability to drive proliferation in thymocytes, and the recombinant guinea pig MCP-1 exhibited chemotactic activity for guinea pig resident peritoneal macrophages. These biologically active recombinant guinea pig proteins will facilitate an in-depth understanding of the role they play in the immune responses of the guinea pig to TB and other diseases.
豚鼠（Cavia porcellus）是研究人类结核病（TB）和许多其他传染性和非传染性疾病的优秀动物模型。有效利用这种动物模型的主要障碍之一是缺乏现成的免疫试剂。为了解决这个问题，我们在原核表达载体中高效克隆和表达了豚鼠白细胞介素 1β（IL-1β）和单核细胞趋化蛋白-1（MCP-1），并通过 N 端测序证实了这两个基因表达的蛋白均以可溶性形式存在。重组豚鼠 IL-1β 的生物学活性通过其促进胸腺细胞增殖的能力得到证实，重组豚鼠 MCP-1 对豚鼠驻留腹腔巨噬细胞具有趋化活性。这些具有生物活性的重组豚鼠蛋白将有助于深入了解它们在豚鼠对 TB 和其他疾病的免疫反应中所起的作用。
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