Center for AIDS Research, Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA.
Tuberculosis (Edinb). 2011 May;91(3):250-6. doi: 10.1016/j.tube.2011.02.006. Epub 2011 Mar 10.
We sought to identify biomarker responses to tuberculosis specific antigens which could 1) improve the diagnosis of tuberculosis infection and 2) allow the differentiation of active and latent infections. Seventy subjects with active tuberculosis (N = 12), latent tuberculosis (N = 32), or no evidence of tuberculosis infection (N = 26) were evaluated. We used the Luminex Multiplexed Bead Array platform to simultaneously evaluate 25 biomarkers in the supernatant of whole blood samples following overnight stimulation using the Quantiferon(®) Gold In-Tube kit. We defined the response to stimulation as the difference (within an individual patient) between the response to the pooled tuberculosis antigens and the negative control. IP-10 response was significantly higher in tuberculosis-infected (active or latent) subjects compared to the uninfected group (p < 0.0001). Among the 25 parameters, expression levels of IL-15 and MCP-1 were found to be significantly higher in the active tuberculosis group compared to the latent tuberculosis group (p = 0.0006 and 0.0030, respectively). When combined, IL-15 and MCP-1 accurately identified 83% of active and 88% of latent infections. The combination of IL-15 and MCP-1 responses was accurate in distinguishing persons with active tuberculosis from persons with latent tuberculosis in this study.
我们试图确定对结核分枝杆菌特异抗原的生物标志物反应，这些反应能够 1）改善结核感染的诊断，2）区分活动性和潜伏性感染。我们评估了 70 名活动性肺结核（N=12）、潜伏性肺结核（N=32）或无结核感染证据（N=26）的受试者。我们使用 Luminex 多重珠粒阵列平台，在使用 Quantiferon（®）金管试剂盒过夜刺激后，同时评估全血样本上清液中的 25 种生物标志物。我们将刺激反应定义为个体内对 pooled tuberculosis 抗原的反应与阴性对照之间的差异。与未感染组相比，感染结核分枝杆菌（活动性或潜伏性）的受试者的 IP-10 反应明显更高（p<0.0001）。在 25 个参数中，IL-15 和 MCP-1 的表达水平在活动性肺结核组中明显高于潜伏性肺结核组（p=0.0006 和 0.0030）。联合使用时，IL-15 和 MCP-1 可准确识别 83%的活动性感染和 88%的潜伏性感染。在这项研究中，IL-15 和 MCP-1 反应的组合可准确区分活动性肺结核患者和潜伏性肺结核患者。
