School of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Pharmacy, China Medical University, Taichung, Taiwan.
Am J Chin Med. 2012;40(3):581-97. doi: 10.1142/S0192415X12500449.
We investigated possible mechanisms of analgesic and anti-inflammatory activities of the methanol extract from the leaf of Elaeagnus oldhamii Maxim. (EO(MeOH)). EO(MeOH) was evaluated for its analgesic activity in acetic acid-induced writhing response and formalin test, and anti-inflammatory effect was examined by λ-carrageenan-induced paw edema assay. We detected the activities of GPx, GRd and SOD in the liver, and the levels of inflammatory mediators including IL-1β, IL-6, TNF-α, COX-2, MDA and NO in the edema paw to investigate the mechanism of action against inflammation. Total polyphenol, flavonoid and flavanol contents of EO(MeOH) were detected to explore its antioxidant activities. Results showed that, in the analgesic test, EO(MeOH) decreased acetic acid-induced writhing response and the licking time in the late phase of formalin test. In the anti-inflammatory test, EO(MeOH) decreased paw edema at the 2nd, 3rd, 4th and 5th h after λ-carrageenan had been injected. EO(MeOH) increased the activities of SOD and GPx in liver tissue and decreased MDA, NO, IL-1β, IL-6, TNF-α and COX-2 levels in paw edema tissue at the 3rd h after λ-carrageenan-induced inflammatory reaction. EO(MeOH) exhibited abundant polyphenol, flavonoid and flavanol contents. In HPLC fingerprint test of EO(MeOH), two index ingredients, ursolic acid and pomolic acid, were isolated from EO(MeOH) and were exhibited in HPLC chromatographic analysis. The results demonstrated analgesic and anti-inflammatory effects of EO(MeOH). It was indicated that the anti-inflammatory mechanism of EO(MeOH) may be due to declined levels of NO and MDA in the edema paw through increasing the activities of SOD, GPx and GRd in the liver. Additionally, EO(MeOH) decreased IL-1β, IL-6, TNF-α and COX-2 levels in the edema paw. The results suggested its value in future development of herbal medicine for the treatment of inflammatory diseases.
我们研究了沙枣叶甲醇提取物（EO(MeOH)）的镇痛和抗炎活性的可能机制。通过醋酸诱导的扭体反应和福尔马林试验评价 EO(MeOH)的镇痛活性，通过 λ-卡拉胶诱导的足肿胀试验检测抗炎作用。我们检测了肝脏中 GPx、GRd 和 SOD 的活性，以及肿胀足中包括 IL-1β、IL-6、TNF-α、COX-2、MDA 和 NO 在内的炎症介质的水平，以研究其抗炎作用机制。检测 EO(MeOH)中的总多酚、类黄酮和黄烷醇含量，以探讨其抗氧化活性。结果表明，在镇痛试验中，EO(MeOH)减少了醋酸诱导的扭体反应和福尔马林试验后期的舔足时间。在抗炎试验中，EO(MeOH)减少了 λ-卡拉胶注射后第 2、3、4 和 5 小时的足肿胀。EO(MeOH)增加了肝脏组织中 SOD 和 GPx 的活性，并降低了 MDA、NO、IL-1β、IL-6、TNF-α和 COX-2 水平在 λ-卡拉胶诱导的炎症反应后 3 小时的肿胀足组织中。EO(MeOH)显示出丰富的多酚、类黄酮和黄烷醇含量。在 EO(MeOH)的 HPLC 指纹图谱试验中，从 EO(MeOH)中分离出两种指标成分熊果酸和齐墩果酸，并在 HPLC 色谱分析中得到了展示。结果表明 EO(MeOH)具有镇痛和抗炎作用。表明 EO(MeOH)的抗炎机制可能是通过增加肝脏中 SOD、GPx 和 GRd 的活性，降低肿胀足中 MDA 和 NO 的水平。此外，EO(MeOH)降低了肿胀足中的 IL-1β、IL-6、TNF-α和 COX-2 水平。结果表明，它在未来开发草药治疗炎症性疾病方面具有价值。
