Chinese Field Epidemiology Training Program, Chinese Center for Disease Control and Prevention, Beijing, China.
PLoS One. 2012;7(6):e38712. doi: 10.1371/journal.pone.0038712. Epub 2012 Jun 20.
Clusters of sudden unexplained death (SUD) in Yunnan Province, China, have been linked to eating Trogia venenata mushrooms. We evaluated the toxic effect of this mushroom on mice.
We prepared extracts of fresh T. venenata and Laccaria vinaceoavellanea mushrooms collected from the environs of a village that had SUD. We randomly allocated mice into treatment groups and administered mushroom extracts at doses ranging from 500 to 3500 mg/kg and water (control) via a gavage needle. We observed mice for mortality for 7 days after a 3500 mg/kg dose and for 24 hours after doses from 500 to 3000 mg/kg. We determined biochemical markers from serum two hours after a 2000 mg/kg dose.
Ten mice fed T. venenata extract (3500 mg/kg) died by five hours whereas all control mice (L. vinaceoavellanea extract and water) survived the seven-day observation period. All mice died by five hours after exposure to single doses of T. venenata extract ranging from 1500 to 3000 mg/kg, while the four mice exposed to a 500 mg/kg dose all survived. Mice fed 2000 mg/kg of T. venenata extract developed profound hypoglycemia (median= 0.66 mmol/L) two hours after exposure.
Hypoglycemia and death within hours of exposure, a pattern unique among mushroom toxicity, characterize T. venenata poisoning.
在中国云南省，簇状不明原因猝死（SUD）与食用 Trogia venenata 蘑菇有关。我们评估了这种蘑菇对小鼠的毒性作用。
我们从发生 SUD 的村庄周围采集新鲜 T. venenata 和 Laccaria vinaceoavellanea 蘑菇，制备提取物。我们将小鼠随机分配到治疗组，通过胃管针给予 500 至 3500mg/kg 剂量的蘑菇提取物和水（对照）。在给予 3500mg/kg 剂量后 7 天和 500 至 3000mg/kg 剂量后 24 小时观察小鼠的死亡率。在给予 2000mg/kg 剂量后 2 小时从血清中测定生化标志物。
10 只喂食 T. venenata 提取物（3500mg/kg）的小鼠在 5 小时内死亡，而所有对照小鼠（L. vinaceoavellanea 提取物和水）在 7 天观察期内均存活。所有小鼠在暴露于 1500 至 3000mg/kg 剂量的 T. venenata 提取物后 5 小时内死亡，而暴露于 500mg/kg 剂量的 4 只小鼠全部存活。喂食 2000mg/kg T. venenata 提取物的小鼠在暴露后 2 小时出现严重低血糖（中位数=0.66mmol/L）。
暴露后数小时内发生低血糖和死亡，这是蘑菇毒性中的一种独特模式，是 T. venenata 中毒的特征。
