利用小牛胸腺部分纯化的核苷酸激酶合成糖修饰的核苷5'-三磷酸。

Synthesis of sugar-modified nucleoside 5'-triphosphates with partially purified nucleotide kinases from calf thymus.

作者信息

Imazawa M, Eckstein F

出版信息

Biochim Biophys Acta. 1979 Oct 11;570(2):284-90. doi: 10.1016/0005-2744(79)90148-7.

Abstract

The partial purification of some nucleoside monophosphate kinases (ATP:nucleosidemonophosphate phosphotransferases, EC 2.7.4.4) from calf thymus by chromatography on Blue Sepharose to remove interfering phosphatase activity is described. Their specificities towards nucleoside monophosphates modified in the sugar are investigated. Pyrimidine nucleoside monophosphate kinase is not very much affected by such modifications, whereas GMP kinase does not tolerate such alteration. The effect on AMP kinase is intermediate.

摘要

描述了通过在蓝色琼脂糖凝胶上进行色谱分离以去除干扰性磷酸酶活性，从小牛胸腺中部分纯化一些核苷单磷酸激酶（ATP：核苷单磷酸磷酸转移酶，EC 2.7.4.4）的方法。研究了它们对糖基修饰的核苷单磷酸的特异性。嘧啶核苷单磷酸激酶受此类修饰的影响不大，而GMP激酶则不能耐受这种改变。对AMP激酶的影响介于两者之间。