Centre for Environment, Fisheries and Aquaculture Science, Pakefield Road, Lowestoft, Suffolk NR33 OHT, U.K.
J Fish Biol. 2012 Jul;81(1):320-8. doi: 10.1111/j.1095-8649.2012.03298.x. Epub 2012 May 22.
Experiments were conducted on the ability of TaqMan molecular probes to detect plaice Pleuronectes platessa DNA from eggs, and cod Gadus morhua DNA from eggs and larvae following ingestion by a teleost predator, whiting Merlangius merlangus. Estimated half-life detection rate (T50) for eggs was 31 h, and 26 h for larvae, with some positive detections occurring even after visual inspection indicated complete gut clearance. Because TaqMan probes are taxon specific, the results presented demonstrate that this technique can provide a means of rapid and unambiguous detection of predation by teleosts on fish eggs and larvae.
进行了实验，以评估 TaqMan 分子探针检测鲽鱼 Pleuronectes platessa 卵 DNA 和鳕鱼 Gadus morhua 卵及幼虫 DNA 的能力，这些卵及幼虫是被一种硬骨鱼掠食者——牙鳕 Merlangius merlangus 吞食后的残留。卵的估计半衰期检测率（T50）为 31 小时，幼虫为 26 小时，即使在肉眼观察表明已完全排空肠道后，仍有一些阳性检测结果。由于 TaqMan 探针是分类特异性的，因此所呈现的结果表明，该技术可提供一种快速、明确的方法，用于检测硬骨鱼对鱼类卵和幼虫的捕食。
