电磁脉冲辐射对小鼠血睾屏障的影响。
Effects of electromagnetic pulse irradiation on the mouse blood-testicle barrier.
机构信息
Department of Anesthesiology, Xijing Hospital, Xi'an, China.
出版信息
Urology. 2012 Jul;80(1):225.e1-6. doi: 10.1016/j.urology.2012.04.015.
OBJECTIVE
To investigate the effects of electromagnetic pulse irradiation on the mouse blood-testicle barrier (BTB) and spermatogenesis.
METHODS
After whole body irradiation with 400 kV/m electromagnetic pulse irradiation, the mouse testicles and BTB permeability were observed using hematoxylin-eosin, Evans blue, and lanthanum nitrate as tracers. The expression of the BTB tight junction protein occludin was examined using real-time polymerase chain reaction and Western blotting.
RESULTS
At 1, 7, and 14 days after irradiation, the BTB structure was damaged, the BTB permeability was significantly increased, numerous apoptotic or necrotic spermatogenic cells were found in the lumen, and the mRNA and protein expression levels of occludin were markedly decreased. The BTB structure and occludin expression levels had gradually recovered by 21 and 28 days after irradiation.
CONCLUSION
Electromagnetic pulse irradiation damaged the structure and function of mouse BTB, resulting in apoptosis or necrosis of the spermatogenic cells.
目的
研究电磁脉冲辐射对小鼠血睾屏障（BTB）和生精作用的影响。
方法
采用 400kV/m 电磁脉冲全身照射后，利用苏木精-伊红、伊文思蓝和硝酸镧示踪剂观察小鼠睾丸和 BTB 通透性，实时聚合酶链反应和 Western blot 检测 BTB 紧密连接蛋白 occludin 的表达。
结果
照射后 1、7 和 14 天，BTB 结构受损，BTB 通透性明显增加，管腔中出现大量凋亡或坏死的生精细胞，occludin 的 mRNA 和蛋白表达水平明显降低。照射后 21 和 28 天，BTB 结构和 occludin 表达水平逐渐恢复。
结论
电磁脉冲辐射破坏了小鼠 BTB 的结构和功能，导致生精细胞凋亡或坏死。
Zotero 插件