墨西哥原发性血栓形成倾向 IX 研究：糖蛋白 IIIa PLA1/A2 多态性与黏附血小板综合征表型无关。
Primary thrombophilia in Mexico IX: the glycoprotein IIIa PLA1/A2 polymorphism is not associated with the sticky platelet syndrome phenotype.
机构信息
1Centro de Hematología y Medicina Interna de Puebla, Mexico.
出版信息
Clin Appl Thromb Hemost. 2013 Nov-Dec;19(6):689-92. doi: 10.1177/1076029612448418. Epub 2012 Jun 29.
INTRODUCTION
The sticky platelet syndrome (SPS) seems to be a common cause of thrombosis, although no molecular substrate to explain platelet hyperaggregability has been found.
OBJECTIVE
To analyze an association between the SPS phenotype and the platelet glycoprotein (GP) IIIa PL(A1/A2) (human platelet antigen [HPA]-1a/b) gene polymorphism.
METHODS
Along an 18-month period, Mexican mestizo thrombophilic patients were prospectively accrued. The SPS phenotype was assessed by aggregometry, whereas a tetra-primer amplification refractory mutation system (ARMS) polymerase chain reaction analysis was used to detect the PLA1 and PLA2 alleles.
RESULTS
A total of 95 individuals with SPS and 127 healthy donors were studied; in 11 of the donors and 16 of the patients with SPS the A2 allele of the GP IIb/IIIA was found, yielding a weak and nonsignificant association (odds ratio 2.14, 95% CI 0.94-4.85).
CONCLUSION
In Mexican mestizo patients, the platelet GP IIIa PL(A1/A2) gene polymorphism does not lead to the SPS phenotype.
简介
粘性血小板综合征（SPS）似乎是血栓形成的常见原因，尽管尚未发现可解释血小板高聚集性的分子基础。
目的
分析 SPS 表型与血小板糖蛋白（GP）IIIa PL(A1/A2)（人类血小板抗原[HPA]-1a/b）基因多态性之间的关联。
方法
在 18 个月的时间内，前瞻性地招募了墨西哥混血血栓形成倾向患者。通过聚合酶链反应分析聚合酶链反应分析来评估 SPS 表型，而四引物扩增耐突变系统（ARMS）聚合酶链反应分析用于检测 PLA1 和 PLA2 等位基因。
结果
共研究了 95 名 SPS 患者和 127 名健康献血者；在 11 名献血者和 16 名 SPS 患者中发现了 GP IIb/IIIa 的 A2 等位基因，这与较弱且无统计学意义的相关性（比值比 2.14，95%置信区间 0.94-4.85）。
结论
在墨西哥混血患者中，血小板 GP IIIa PL(A1/A2)基因多态性不会导致 SPS 表型。
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