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破坏编码单 ADP-核糖基转移酶的 SCO5461 基因会产生条件性的多效表型,影响链霉菌形态分化和抗生素的产生。

Disruption of SCO5461 gene coding for a mono-ADP-ribosyltransferase enzyme produces a conditional pleiotropic phenotype affecting morphological differentiation and antibiotic production in Streptomyces coelicolor.

机构信息

Department of Human Genetics, Medical and Health Science Center, University of Debrecen, Debrecen, Nagyerdei krt 98, H-4032, Hungary.

出版信息

J Microbiol. 2012 Jun;50(3):409-18. doi: 10.1007/s12275-012-1440-y. Epub 2012 Jun 30.

Abstract

The SCO5461 gene of Streptomyces coelicolor A3(2) codes for an ADP-ribosyltransferase enzyme that is predicted to be a transmembrane protein with an extracellular catalytic domain. PCR-targeted disruption of the gene resulted in a mutant that differentiated normally on complex SFM medium; however, morphological differentiation in minimal medium was significantly delayed and this phenotype was even more pronounced on osmotically enhanced minimal medium. The mutant did not sporulate when it was grown on R5 medium, however the normal morphological differentiation was restored when the strain was cultivated beside the wild-type S. coelicolor M145 strain. Comparison of the pattern of ADP-ribosylated proteins showed a difference between the mutant and the wild type, fewer modified proteins were present in the cellular crude extract of the mutant strain. These results support our previous suggestions that protein ADP-ribosylation is involved in the regulation of differentiation and antibiotic production and secretion in Streptomyces.

摘要

链霉菌 A3(2)的 SCO5461 基因编码一种 ADP-核糖基转移酶,该酶预测为一种跨膜蛋白,具有细胞外催化结构域。该基因的 PCR 靶向缺失导致突变体在复杂 SFM 培养基中正常分化;然而,在最小培养基中的形态分化明显延迟,在渗透压增强的最小培养基中这种表型更为明显。当突变体在 R5 培养基中生长时,它不会产孢,然而当菌株在野生型链霉菌 M145 菌株旁边培养时,正常的形态分化得到恢复。ADP-核糖基化蛋白的模式比较表明突变体和野生型之间存在差异,突变菌株的细胞粗提取物中存在的修饰蛋白较少。这些结果支持我们之前的假设,即蛋白质 ADP-核糖基化参与链霉菌分化和抗生素产生和分泌的调控。

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