Xie Jiansong, Ferbas John, Juan Gloria
Department of Medical Sciences, Amgen, Thousand Oaks, California, USA.
Curr Protoc Cytom. 2012 Jul;Chapter 6:Unit 6.33. doi: 10.1002/0471142956.cy0633s61.
Multiphoton-induced second-harmonic generation (SHG) has developed into a very powerful approach for in depth visualization of some biological structures with high specificity. In this unit, we describe the basic principles of three-dimensional SHG microscopy. In addition, we illustrate how SHG imaging can be utilized to assess collagen fibrils in biological tissues. Some technical considerations are also addressed.
多光子诱导二次谐波产生（SHG）已发展成为一种非常强大的方法，可用于高特异性地深入可视化某些生物结构。在本单元中，我们描述了三维SHG显微镜的基本原理。此外，我们还说明了如何利用SHG成像来评估生物组织中的胶原纤维。同时还讨论了一些技术方面的注意事项。
