Koistinaho J, Wadhwani K C, Latker C H, Balbo A, Rapoport S I
Laboratory of Neurosciences, National Institute on Aging, National Institutes of Health, Bethesda, MD 20892.
Acta Neuropathol. 1990;80(6):604-10. doi: 10.1007/BF00307627.
Adrenergic innervation of blood vessels in rat tibial nerve during Wallerian degeneration was examined, using the formaldehyde-induced histo-fluorescence method. The left sciatic nerve was transected at the level of the sciatic notch, whereas the right sciatic nerve was left intact and used as control. At 1, 3, 7, 14, 42, 56 or 84 days after transection, the tibial nerves of the transected and contralateral sides were exposed. Pieces of each nerve were used for light microscopy or for examination of adrenergic innervation with the fluorescence microscope. One day after transection, no adrenergic nerve fiber was observed in the endoneurium of the transected nerve. After 3 days, adrenergic innervation of small- and medium-sized arterioles in the epi-perineurium was absent, and after 7 days no fibers were visible around large arterioles. Fluorescent fibers were not detected even at 84 days post-surgery. It is concluded that adrenergic innervation of blood vessels in the rat tibial nerve is irreversibly lost after permanent axotomy, and that adrenergic regulation of nerve blood flow may also be lost.
采用甲醛诱导组织荧光法，对大鼠胫神经在华勒氏变性过程中血管的肾上腺素能神经支配进行了研究。将左侧坐骨神经在坐骨切迹水平切断，右侧坐骨神经保持完整作为对照。在切断后1、3、7、14、42、56或84天，暴露切断侧和对侧的胫神经。每条神经的片段用于光学显微镜检查或用荧光显微镜检查肾上腺素能神经支配。切断后1天，在切断神经的神经内膜中未观察到肾上腺素能神经纤维。3天后，在神经外膜中小动脉的肾上腺素能神经支配消失，7天后在大动脉周围未见纤维。即使在术后84天也未检测到荧光纤维。结论是，大鼠胫神经血管的肾上腺素能神经支配在永久性轴突切断后不可逆转地丧失，并且神经血流的肾上腺素能调节也可能丧失。
