用于龙舌兰属帕里龙舌兰和珀耳氏龙舌兰（龙舌兰科）的新型微卫星位点及其交叉扩增。

Novel microsatellite loci for Agave parryi and cross-amplification in Agave palmeri (Agavaceae).

机构信息

Environmental Laboratory, U.S. Army Engineer Research and Development Center, 3909 Halls Ferry Road, Vicksburg, MS 39180, USA.

出版信息

Am J Bot. 2012 Jul;99(7):e295-7. doi: 10.3732/ajb.1200033. Epub 2012 Jul 2.

DOI:10.3732/ajb.1200033

PMID:22753814

Abstract

PREMISE OF THE STUDY

To examine the foraging behavior of nectarivorous bats in southeastern Arizona, we developed microsatellite primers in Agave parryi. These markers were also tested for cross-amplification and applicability to assess patterns of genetic diversity and structure in A. palmeri.

METHODS AND RESULTS

Utilizing DNA sequence data from 454 shotgun sequencing, we identified seven novel polymorphic microsatellite loci in A. parryi and screened them for cross-amplification in A. palmeri. These markers were characterized in two populations of 30 individuals each for each species. In A. parryi, all primers were polymorphic and amplified between three and 12 alleles per population. In A. palmeri, all primers amplified, six were polymorphic, and allelic diversity ranged from one to 16 alleles per population.

CONCLUSIONS

Our results demonstrate the applicability of these microsatellite primers for population genetics studies in both A. parryi and A. palmeri.

摘要

研究前提

为了研究亚利桑那州东南部食蜜蝙蝠的觅食行为，我们在龙舌兰属植物中开发了微卫星引物。这些标记物也经过了交叉扩增测试，适用于评估 A. palmeri 遗传多样性和结构的模式。

方法和结果

利用来自 454 shotgun 测序的 DNA 序列数据，我们在龙舌兰属植物中鉴定了七个新的多态性微卫星位点，并在 A. palmeri 中筛选了这些位点的交叉扩增。这些标记物在每个物种的 30 个个体的两个种群中进行了特征描述。在龙舌兰属植物中，所有引物都是多态的，每个种群扩增出 3 到 12 个等位基因。在 A. palmeri 中，所有引物都能扩增，其中 6 个是多态的，每个种群的等位基因多样性范围从 1 到 16 个等位基因。