University of Zagreb, Faculty of Science, Division of Biology, Department of Botany, Marulicev trg 9a, HR 10000 Zagreb, Croatia.
Am J Bot. 2011 Nov;98(11):e316-8. doi: 10.3732/ajb.1000462. Epub 2011 Oct 14.
Microsatellite primers were identified to study the genetic diversity, population genetic structure, and relationships of common sage and closely related species.
Nine novel polymorphic trinucleotide microsatellite loci were identified for Salvia officinalis. A total of 127 alleles were observed. The observed and expected heterozygosity values ranged from 0.375 to 0.880 and from 0.624 to 0.931, respectively. Nine new and 11 previously published microsatellite primers were tested for cross-amplification. The species with the most successful cross-amplification was S. fruticosa.
The nine new microsatellite markers will be useful in genetic studies of wild and cultivated populations of common sage and are potentially useful in genetic studies of closely related species.
鉴定了微卫星引物，以研究普通鼠尾草和近缘种的遗传多样性、种群遗传结构和关系。
为 Salvia officinalis 鉴定了 9 个新的多态性三核苷酸微卫星位点。共观察到 127 个等位基因。观察到的和预期的杂合度值分别在 0.375 到 0.880 和 0.624 到 0.931 之间。9 个新的和 11 个以前发表的微卫星引物被测试用于交叉扩增。交叉扩增最成功的物种是 S. fruticosa。
这 9 个新的微卫星标记将有助于对普通鼠尾草的野生和栽培种群进行遗传研究，并且可能对近缘种的遗传研究有用。
