Park Juhyun, Kim Hyo-Yeon, Lee Sun-Mee
School of Pharmacy, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
Afr J Tradit Complement Altern Med. 2011;8(5 Suppl):220-5. doi: 10.4314/ajtcam.v8i5S.2. Epub 2011 Jul 3.
This study evaluated the potential beneficial effect of Moutan Cortex Radicis (MCR) in a murine model of carbon tetrachloride (CCl(4))-, D-galactosamine (GalN)- and α-naphthylisothiocyanate (ANIT)-induced liver injury. Acute hepatotoxicity was induced by intraperitoneal injection of CCl(4) (10 µL/kg), GalN (700 mg/kg), and ANIT (40 mg/kg). Animals received MCR (30, 100, and 300 mg/kg ) orally at 48, 24, and 2 h before and 6 h after administration of CCl(4), GalN, and ANIT. Serum activities of aminotransferase were significantly higher at 24 h after CCl(4) or GalN treatment. These changes were attenuated by MCR. Histopathological analysis revealed multiple and extensive areas of portal inflammation, hepatocellular necrosis, and an increase in inflammatory cell infiltration. These changes were inhibited by MCR. Serum total bilirubin concentration increased and bile flow decreased significantly 48 h after ANIT treatment, which was attenuated by MCR. Our results suggest that MCR has a protective effect on acute liver injury.
本研究评估了牡丹皮（MCR）在四氯化碳（CCl₄）、D-半乳糖胺（GalN）和α-萘异硫氰酸酯（ANIT）诱导的小鼠肝损伤模型中的潜在有益作用。通过腹腔注射CCl₄（10 μL/kg）、GalN（700 mg/kg）和ANIT（40 mg/kg）诱导急性肝毒性。在给予CCl₄、GalN和ANIT之前48、24和2小时以及之后6小时，动物口服MCR（30、100和300 mg/kg）。在CCl₄或GalN处理后24小时，血清转氨酶活性显著更高。这些变化被MCR减弱。组织病理学分析显示门静脉炎症、肝细胞坏死的多个广泛区域以及炎症细胞浸润增加。这些变化被MCR抑制。ANIT处理后48小时，血清总胆红素浓度升高且胆汁流量显著降低，这被MCR减弱。我们的结果表明MCR对急性肝损伤具有保护作用。
