中华苦荬菜沸水提取物对乙型肝炎病毒活性和肝细胞癌的影响
Effects of Ixeris Chinensis (Thunb.) Nakai boiling water extract on hepatitis B viral activity and hepatocellular carcinoma.
作者信息
Shih Ko-Nien, Huang Wen-Tao, Chang Chung-Liang, Feng Chih-Chung
机构信息
Department of Radiological Technology, College of Medical Sciences and Technology, Yuanpei University, HsinChu City 30015, Taiwan.
Department of Environmental Engineering and Health, College of Health Sciences, Yuanpei University, HsinChu City 30015, Taiwan.
出版信息
Afr J Tradit Complement Altern Med. 2013 Nov 2;11(1):187-93. doi: 10.4314/ajtcam.v11i1.30. eCollection 2014.
BACKGROUND
Hepatitis B virus (HBV) infection and hepatocellular carcinoma are major diseases that affect the Taiwanese population. Therefore, the development of an alternative herbal medicine that can effectively treat these diseases is a research target. In this study, we tested Ixeris Chinensis (Thunb.) Nakai boiling water extract (ICTN BWE) in vitro and analysed its effects on the HBV and liver cancer.
MATERIALS AND METHODS
We used a human liver cancer cell line (Hep3B, a cell line that continuously secretes HBV particles into a medium) as an experimental model for the screening of various ICTN BWE concentrations and their effects on the HBV in vitro.
RESULTS
Our results showed that 75 µg/mL ICTN BWE downregulated the relative expression of the hepatitis B virus surface antigens (HBsAg) to 77.1%. Using the human liver cancer cell lines HuH-7 and HepG2, and 3-(4,5-dimethylthiazol-zyl)-2,5-diphenyl tetrazolium bromide (MTT) and tumour clonogenic assays, we then showed that ICTN BWE inhibits hepatocellular carcinoma growth.
CONCLUSION
Fluorescent microscopy of DAPI(4',6-Diamidino-2-phenylindole)-stained nuclei and DNA fragmentation assays confirmed the inhibitory effects of ICTN BWE on liver tumour cell growth through induction of apoptosis.
背景
乙型肝炎病毒（HBV）感染和肝细胞癌是影响台湾人群的主要疾病。因此，开发一种能有效治疗这些疾病的替代草药是一个研究目标。在本研究中，我们在体外测试了中华苦荬菜沸水提取物（ICTN BWE），并分析了其对HBV和肝癌的影响。
材料与方法
我们使用人肝癌细胞系（Hep3B，一种能持续向培养基中分泌HBV颗粒的细胞系）作为实验模型，筛选不同浓度的ICTN BWE及其对体外HBV的影响。
结果
我们的结果表明，75μg/mL的ICTN BWE可将乙型肝炎病毒表面抗原（HBsAg）的相对表达下调至77.1%。然后，我们使用人肝癌细胞系HuH-7和HepG2，以及3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐（MTT）和肿瘤克隆形成试验，证明ICTN BWE可抑制肝癌细胞生长。
结论
用4',6-二脒基-2-苯基吲哚（DAPI）染色细胞核的荧光显微镜检查和DNA片段化试验证实，ICTN BWE通过诱导凋亡对肝肿瘤细胞生长具有抑制作用。
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