Macías-Reyes Héctor, Ramos-Zúñiga Rodrigo, Garcia-Estrada Joaquín, Jáuregui-Huerta Fernando, Hidalgo-Mariscal Maria Leticia
Department of Otorhinolaryngology, Hospital Civil Fray A, Alcalde, Calderón, Col. El Retiro, Jalisco, Mexico.
Surg Neurol Int. 2012;3:68. doi: 10.4103/2152-7806.97537. Epub 2012 Jun 19.
Experimental procedures will continue to be a key element while going through the learning curve in the use of the endoscope and minimally invasive procedures. We describe the technical procedure of an experimental approach to middle ear in New Zealand rabbits through external auditory canal and its relevance as an ideal model to study graft materials and serve as a training tool for potential applications in otoneurology.
A group of 28 adult New Zealand rabbits were subjected to an experimental myringoplasty, combining the transmeatal and retroauricular approach with endoscopic assistance and microsurgical technique. The different anatomical steps and systematization of the complete experimental procedure are described.
An experimental approach to middle ear live model and basic anatomic description was successfully used, standardizing the ideal technique. The eardrum could regenerate with no complications and with functional preservation in all the myringoplasty cases. This strategy involves a safe combined approach to the tympanic membrane and others neurosurgical as transcochlear and translaberyntic approaches and is useful as a test of other experimental procedures to evaluate biomaterials to repair the eardrum currently studied. This experimental myringoplasty model also facilitates functional tests such as impedanciometry and the endoscopic follow-up of the whole process.
The method described to perform an experimental myringoplasty (type I tympanoplasty) in a New Zealand rabbit is an option to be used as a basic model to study the behavior of the graft in the tympanic membrane. Also, basic concepts for the use of combined instrumentation are established in the treatment of eardrum lesions, as a refinement of the technical training application in microsurgery and assisted endoscopy in the transcochlear and translaberintic approaches and otoneurology areas.
在掌握内窥镜及微创手术的使用技巧、经历学习曲线的过程中,实验操作仍是关键要素。我们描述了一种通过外耳道对新西兰兔中耳进行实验性操作的技术方法,以及该方法作为研究移植材料的理想模型和耳神经学潜在应用培训工具的相关性。
对28只成年新西兰兔进行实验性鼓膜成形术,采用经耳道和耳后入路并借助内窥镜辅助及显微外科技术。描述了完整实验过程的不同解剖步骤及系统化方法。
成功运用了一种中耳活体模型的实验方法及基本解剖描述,规范了理想技术。在所有鼓膜成形术病例中,鼓膜均可再生且无并发症,功能得以保留。该策略涉及一种安全的联合入路,可用于鼓膜及其他神经外科手术,如经耳蜗和经迷路入路,并且作为评估目前正在研究的用于修复鼓膜的生物材料的其他实验操作的测试也很有用。这种实验性鼓膜成形术模型还便于进行诸如阻抗测量等功能测试以及对整个过程的内窥镜随访。
所描述的在新西兰兔身上进行实验性鼓膜成形术(I型鼓室成形术)的方法,可作为研究鼓膜移植行为的基础模型。此外,在鼓膜病变治疗中确立了联合器械使用的基本概念,作为在经耳蜗和经迷路入路及耳神经学领域显微手术和辅助内窥镜技术培训应用的改进。