Department of Forest Sciences, Seoul National University, Seoul, South Korea.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2012;47(12):1878-88. doi: 10.1080/03601234.2012.676432.
Polychlorinated biphenyls (PCBs) are difficult to degrade due to poor solubility, toxicity, and thermal stability. In the present study, the feasibility of PCB congener biodegradation by Ceriporia sp. ZLY-2010 was evaluated. The biodegradation rates of four PCB congeners, 4,4'-dichlorobiphenyl, 2,3',4',5-tetrachlorobiphenyl, 2,2',4,5,5'-pentachlorobiphenyl, and 2,2',4,4',5,5'-hexachlorobiphenyl were evaluated. The degradation rate of 4,4'-dichlorobiphenyl was 34.03% on incubation day 13, while that of 2,2',4,4',5,5'-hexachlorobiphenyl reached 40.05% on incubation day 17. Therefore, Ceriporia sp. ZLY-2010 was degrading the higher PCB congeners more efficiently. PCB congener degradation products were extracted using acetone and ethyl acetate. No 2,2',4,5,5'-pentachlorobiphenyl metabolites were detected in Ceriporia sp. ZLY-2010 culture, whereas 2,2',4,4',5,5'-hexachlorobiphenyl appeared to degrade to benzoic acid. However, intermediates of 2,2',4,4',5,5'-hexachlorobiphenyl were not detected during degradation. Therefore, additional studies should be performed to explore the mechanisms of PCB degradation. Our results indicate that Ceriporia sp. ZLY-2010 is able to degrade highly chlorinated biphenyls and has potential for use in PCB biodegradation and bioremediation.
多氯联苯（PCBs）由于溶解度低、毒性大、热稳定性差，难以降解。本研究评估了 Ceriporia sp. ZLY-2010 对 PCB 同系物生物降解的可行性。评估了 4 种 PCB 同系物，即 4,4'-二氯联苯、2,3',4',5-四氯联苯、2,2',4,5,5'-五氯联苯和 2,2',4,4',5,5'-六氯联苯的生物降解率。在培养第 13 天时，4,4'-二氯联苯的降解率为 34.03%，而在培养第 17 天时，2,2',4,4',5,5'-六氯联苯的降解率达到 40.05%。因此，Ceriporia sp. ZLY-2010 更有效地降解了较高的 PCB 同系物。用丙酮和乙酸乙酯提取 PCB 同系物降解产物。在 Ceriporia sp. ZLY-2010 培养物中未检测到 2,2',4,5,5'-五氯联苯代谢物，而 2,2',4,4',5,5'-六氯联苯似乎降解为苯甲酸。然而，在降解过程中未检测到 2,2',4,4',5,5'-六氯联苯的中间体。因此，应该进行更多的研究来探索 PCB 降解的机制。我们的结果表明，Ceriporia sp. ZLY-2010 能够降解高度氯化的联苯，具有在 PCB 生物降解和生物修复中的应用潜力。
