Department of Forest Sciences, Seoul National University, Seoul, South Korea.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2013;48(10):1280-91. doi: 10.1080/10934529.2013.777242.
This study examined the interrelation between the biodegradation of polychlorinated biphenyls (PCBs) by Ceriporia sp. ZLY-2010 and its fungal enzyme systems. The degradation rates of Aroclor 1254 and 1260 were 29.01% on day 5 and 36.80% on day 10, respectively. MnP (Manganese dependent peroxidase) and laccase activities showed the greatest increases in the samples containing Aroclors, indicating that extracellular enzymes of Ceriporia sp. ZLY-2010 were affected by the addition of Aroclors. However, the relationship between the biodegradation rate and extracellular enzymes might be obscured by the complexity of the biodegradation process. Cytochrome P450 monooxygenase was inhibited and the biodegradation rate of the Aroclor decreased by adding the inhibitor 1-aminobenzotriazole. Two-dimensional gel electrophoresis showed that intracellular enzymes play a significant role in the biodegradation of Aroclor. Complex extracellular and intracellular enzyme systems in Ceriporia sp. ZLY-2010 play an important role in degrading PCBs. Physiological changes of Ceriporia sp. ZLY-2010 caused by PCBs appeared to affect biodegradation of PCBs. However, it is necessary to further study the unidentified enzymes related to the biodegradation of Aroclor.
本研究考察了 Ceriporia sp. ZLY-2010 对多氯联苯（PCBs）的生物降解与其真菌酶系统之间的相互关系。在第 5 天和第 10 天，多氯联苯 Aroclor 1254 和 1260 的降解率分别为 29.01%和 36.80%。在含有 Aroclors 的样品中，MnP（锰依赖过氧化物酶）和漆酶活性显示出最大的增加，表明 Ceriporia sp. ZLY-2010 的细胞外酶受到 Aroclors 的添加影响。然而，生物降解率与细胞外酶之间的关系可能会因生物降解过程的复杂性而变得模糊。添加抑制剂 1-氨基苯并三唑会抑制细胞色素 P450 单加氧酶并降低 Aroclor 的生物降解率。二维凝胶电泳显示，细胞内酶在 Aroclor 的生物降解中起重要作用。ZL2010 中的复杂细胞外和细胞内酶系统在降解 PCBs 中起着重要作用。PCBs 对 Ceriporia sp. ZLY-2010 的生理变化似乎会影响 PCB 的生物降解。然而，有必要进一步研究与 Aroclor 生物降解相关的未识别酶。
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