人类胶质瘤中的肿瘤特异性DNA序列。

Tumor-specific DNA sequences in human gliomas.

作者信息

Cuatico W, Cho J R

出版信息

Cancer. 1979 Oct;44(4):1309-14. doi: 10.1002/1097-0142(197910)44:4<1309::aid-cncr2820440422>3.0.co;2-u.

DOI:10.1002/1097-0142(197910)44:4<1309::aid-cncr2820440422>3.0.co;2-u

Abstract

Utilizing the technique of hydroxyapatite chromatography, normal cellular DNAs were used to recycle off the repeat or normal sequences found in [3H]DNA copied off 70S RNA from malignant astrocytomas. The recycled [3H]DNA were then used to hybridize against DNAs from normal human brain tissues and DNAs from malignant astrocytomas or Grade IV astrocytomas. The results indicated the presence of tumor-specific DNA sequences in malignant astrocytomas, absent in normal brain tissues. The percentages were 88% and 7%, respectively. When recycled medulloblastoma 70S[3H]DNA probes were utilized against DNA'S FROM Grade IV astrocytomas and from normal brain, similar results were obtained. The respective percentages of hybridization were 67% and 7%. Thus it would appear that malignant gliomas contain tumor-specific DNA sequences which are not found in normal brain tissues.

摘要

利用羟基磷灰石层析技术，使用正常细胞DNA从恶性星形细胞瘤的[3H]DNA（从70S RNA复制而来）中回收重复序列或正常序列。然后将回收的[3H]DNA与正常人脑组织的DNA以及恶性星形细胞瘤或IV级星形细胞瘤的DNA进行杂交。结果表明，恶性星形细胞瘤中存在肿瘤特异性DNA序列，而正常脑组织中不存在。其比例分别为88%和7%。当使用回收的髓母细胞瘤70S [3H]DNA探针与IV级星形细胞瘤和正常脑的DNA杂交时，得到了类似的结果。杂交的相应比例分别为67%和7%。因此，恶性胶质瘤似乎含有正常脑组织中未发现的肿瘤特异性DNA序列。