氧自由基和脂质过氧化物对心肌同工酶的影响。
Effect of oxygen free radicals and lipid peroxides on cardiac isoenzymes.
作者信息
Rao P S, Rujikarn N, Weinstein G S, Luber J M, Tyras D H
机构信息
Division of Cardiothoracic Surgery, Long Island Jewish Medical Center, New Hyde Park, NY 11042.
出版信息
Biomed Biochim Acta. 1990;49(6):439-43.
We report here the effect of oxygen free radicals, OFR (superoxide, O2-; hydroxy, OH; t-butylhydroperoxide, H2O2) lipid peroxides (malondialdehyde, MDA), free radical scavengers (superoxide dismutase, catalase, allopurinol) and generator (ferrous chloride) antioxidants (ascorbate, glutathione) spin traps (5,5-dimethyl-1-pyroline-N-oxide, N-t-butyl-L-pheny nitrone) on the cardiac isoenzyme (CK, CK-MB, LDH, LD1) concentrations in the sera of patients with acute myocardial infarction. CK-MB and LD1 were rapidly and completely inactivated by O2- (50 nmol/ml), OH (1 nmol/ml) and MDA (0.6 microM). Butylhydroperoxide (600 microM), and ferrous chloride (200 microM) selectively inhibited CK-MB. The free radical scavengers, antioxidants and spin traps all had minimal effects, and H2O2 had none.
我们在此报告了氧自由基（OFR，超氧化物，O2-；羟基，OH；叔丁基过氧化氢，H2O2）、脂质过氧化物（丙二醛，MDA）、自由基清除剂（超氧化物歧化酶、过氧化氢酶、别嘌呤醇）和自由基生成剂（氯化亚铁）、抗氧化剂（抗坏血酸、谷胱甘肽）、自旋捕捉剂（5,5-二甲基-1-吡咯啉-N-氧化物、N-叔丁基-L-苯基亚硝酮）对急性心肌梗死患者血清中心肌同工酶（CK、CK-MB、LDH、LD1）浓度的影响。CK-MB和LD1可被O2-（50 nmol/ml）、OH（1 nmol/ml）和MDA（0.6 microM）迅速且完全灭活。叔丁基过氧化氢（600 microM）和氯化亚铁（200 microM）可选择性抑制CK-MB。自由基清除剂、抗氧化剂和自旋捕捉剂的影响均极小，而H2O2则无影响。
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