All-Russian Collection of Microorganisms (VKM), G K Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, Russia.
FEMS Microbiol Ecol. 2012 Nov;82(2):501-9. doi: 10.1111/j.1574-6941.2012.01442.x. Epub 2012 Jul 23.
Filamentous fungi in 36 samples of Antarctic permafrost sediments were studied. The samples collected during the Russian Antarctic expedition of 2007-2009 within the framework of the Antarctic Permafrost Age Project (ANTPAGE) were recovered from different depths in ice-free oases located along the perimeter of the continent. Fungal diversity was determined by conventional microbiological techniques combined with a culture-independent method based on the analysis of internal transcribed spacer (ITS2) sequences in total DNA of the samples. The study revealed a rather low fungal population density in permafrost, although the diversity found was appreciable, representing more than 26 genera. Comparison of the data obtained by different techniques showed that the culture-independent method enabled the detection of ascomycetous and basidiomycetous fungi not found by culturing. The molecular method failed to detect members of the genera Penicillium and Cladosporium that possess small-sized spores known to have a high resistance to environmental changes.
对 36 份南极永久冻土沉积物样本中的丝状真菌进行了研究。这些样本是在 2007-2009 年俄罗斯南极考察期间采集的，采集地点位于大陆周边无冰绿洲的不同深度，采集工作是在南极永久冻土时代项目（ANTPAGE）框架内进行的。真菌多样性通过常规微生物学技术与基于样本总 DNA 中内部转录间隔区（ITS2）序列分析的非培养依赖方法相结合来确定。研究结果表明，永久冻土中的真菌种群密度相当低，尽管发现的多样性相当可观，代表了超过 26 个属。不同技术获得的数据比较表明，非培养依赖方法能够检测到通过培养无法检测到的子囊菌和担子菌真菌。分子方法未能检测到具有已知对环境变化具有高抗性的小孢子的青霉属和枝孢属成员。
