Functional changes of the NADH respiratory chain in rat-liver mitochondria and the content changes of high-energy phosphate groups in rat liver and heart during the early phase of burn injury.

Using glutamate plus malate as substrates, the respiratory control ratio of liver mitochondria from burned male Sprague-Dawley rats with full skin thickness burns covering 20 per cent of the body surface area were increased at 45, 60, 75 and 90 min and using alpha-ketoglutarate, were increased at 60 and 75 min after burning, the peak being at 75 min postburn. The ADP/O ratio and the rate of ATP formation were also increased at about 75 min postburn and the ATP content in liver was increased at 120 min following burning. The ATP and creatine phosphate contents in heart tissue were increased at 120 min and at 105, 120 and 135 min postburn respectively. A sham-injured group acted as control. All the results suggested an increased oxidative phosphorylation coupling in some organs during the early phase of burn injury.