Risberg B, Stål O, Eriksson L L, Hussein A
Department of Pathology, Central County Hospital, Orebro, Sweden.
Anal Cell Pathol. 1990 Sep;2(5):287-95.
In this study we have compared two different preparation methods for DNA flow cytometry on breast cancer. Tumour cell suspensions from 49 breast cancers were analysed on a Facscan flow cytometer. In seven of 49 cases, additional aneuploid peaks were found after enzyme/detergent treatment (E/D), not seen after the detergent (D) preparation. S-Phase fractions were significantly higher after D than after E/D preparation (mean values, 15 and 8%, respectively), although the correlation was high between the two methods. S-Phase fraction estimated after background correction diminished the differences between the two methods (mean values, 8 and 6%). Furthermore, the fraction of G2/M cells were generally greater with the D method. These differences can be explained by increased number of cell doublets and nuclear fragments after D compared to E/D preparation. This clearly shows that the preparation method influences the result of DNA flow cytometry on human breast cancers.
在本研究中，我们比较了两种用于乳腺癌DNA流式细胞术的不同制备方法。对49例乳腺癌的肿瘤细胞悬液在Facscan流式细胞仪上进行分析。在49例中的7例中，酶/去污剂处理（E/D）后发现了额外的非整倍体峰，而在去污剂（D）制备后未见到。D法制备后的S期分数显著高于E/D法制备后（平均值分别为15%和8%），尽管两种方法之间的相关性很高。背景校正后估计的S期分数减小了两种方法之间的差异（平均值分别为8%和6%）。此外，D法制备的G2/M期细胞分数通常更大。与E/D法制备相比，这些差异可以通过D法制备后细胞双联体和核碎片数量增加来解释。这清楚地表明，制备方法会影响人类乳腺癌DNA流式细胞术的结果。
