Charpin C, Andrac L, Lavaut M N, Andonian C, Fraterno M, Devictor B, Perez-Castillo A, Bonnier P, Piana L
Department of Pathology, C.H.U. Timone, France.
Anal Cell Pathol. 1990 Oct;2(6):357-71.
DNA nuclear content was assessed in human breast carcinomas (n = 132) using image cytometry. Optical density histograms of Feulgen stained cell imprints from fresh tissue samples, subsequently frozen for immunocytochemical assays, were determined by the SAMBA system and used for the DNA index, the ploidy balance (PB) and the proliferation index (PI) computation. The three parameters were correlated to (i) histological data (tumour grade, vascular and/or lymph node invasion) and to (ii) growth fraction (Ki67), hormone receptor antigenic sites (ER, PR) and intramedullar (bone marrow) biopsies and anti-KL1-positive epithelial cells. It was shown that 57% of breast carcinomas were aneuploid. Aneuploidy PI significantly correlated to the criteria of poor prognosis such as high tumour grade, vascular and lymphatic invasion and to increased Ki67-positive cells, and the absence of or low ER and PR. Since image cytometry is easy to handle and perfectly suitable for current diagnostic practice in pathology departments, particularly for tumour cell ploidy assessment and standardized analysis of immunostaining procedures with morphological control of the preparation, we conclude that image cytometry, as performed with the SAMBA, must be regarded as a relevant tool for prognosis evaluation and therapy guidance in individual patients.
采用图像细胞术评估132例人乳腺癌的DNA核含量。通过SAMBA系统测定新鲜组织样本福尔根染色细胞印片的光密度直方图，这些样本随后被冷冻用于免疫细胞化学分析，并用于计算DNA指数、倍体平衡（PB）和增殖指数（PI）。将这三个参数与（i）组织学数据（肿瘤分级、血管和/或淋巴结浸润）以及（ii）生长分数（Ki67）、激素受体抗原位点（ER、PR）和髓内（骨髓）活检及抗KL1阳性上皮细胞进行相关性分析。结果显示，57%的乳腺癌为非整倍体。非整倍体PI与高肿瘤分级、血管和淋巴管浸润等预后不良标准以及Ki67阳性细胞增加、ER和PR缺失或低表达显著相关。由于图像细胞术易于操作，非常适合病理科当前的诊断实践，特别是用于肿瘤细胞倍体评估以及免疫染色程序的标准化分析并对标本进行形态学对照，我们得出结论，使用SAMBA进行的图像细胞术必须被视为评估个体患者预后和指导治疗的相关工具。
