Instituto de Biología Molecular y Celular de Plantas, CSIC-UPV, Valencia, Spain.
PLoS One. 2012;7(6):e38992. doi: 10.1371/journal.pone.0038992. Epub 2012 Jun 22.
Volatile compounds represent an important part of the plant metabolome and are of particular agronomic and biological interest due to their contribution to fruit aroma and flavor and therefore to fruit quality. By using a non-targeted approach based on HS-SPME-GC-MS, the volatile-compound complement of peach fruit was described. A total of 110 volatile compounds (including alcohols, ketones, aldehydes, esters, lactones, carboxylic acids, phenolics and terpenoids) were identified and quantified in peach fruit samples from different genetic backgrounds, locations, maturity stages and physiological responses. By using a combination of hierarchical cluster analysis and metabolomic correlation network analysis we found that previously known peach fruit volatiles are clustered according to their chemical nature or known biosynthetic pathways. Moreover, novel volatiles that had not yet been described in peach were identified and assigned to co-regulated groups. In addition, our analyses showed that most of the co-regulated groups showed good intergroup correlations that are therefore consistent with the existence of a higher level of regulation orchestrating volatile production under different conditions and/or developmental stages. In addition, this volatile network of interactions provides the ground information for future biochemical studies as well as a useful route map for breeding or biotechnological purposes.
挥发性化合物是植物代谢组的重要组成部分，由于它们对果实香气和风味的贡献，因此对果实品质有重要影响，这使得它们具有特殊的农艺和生物学意义。本研究采用基于 HS-SPME-GC-MS 的非靶向方法，描述了桃果实中的挥发性化合物组成。在不同遗传背景、地理位置、成熟阶段和生理反应的桃果实样本中，共鉴定和定量了 110 种挥发性化合物（包括醇类、酮类、醛类、酯类、内酯类、羧酸类、酚类和萜类）。通过层次聚类分析和代谢组相关网络分析相结合的方法，我们发现以前已知的桃果实挥发性化合物根据其化学性质或已知的生物合成途径聚类。此外，还鉴定出了以前在桃中尚未描述的新挥发性化合物，并将其分配到共同调控的组中。此外，我们的分析表明，大多数共同调控组之间具有良好的组间相关性，因此，在不同条件和/或发育阶段下，存在着更高水平的调控来协调挥发性化合物的产生。此外，该挥发性相互作用网络为未来的生化研究提供了基础信息，以及为育种或生物技术目的提供了有用的路线图。
