Mekkour M, Ben Driss E, Tai J, Squinazi F, Forey F, Jarraud S, Cohen N
Division de Microbiologie et Hygiène des Aliments et de l'Environnement, l'Institut Pasteur du Maroc-Casablanca, Maroc.
Cell Mol Biol (Noisy-le-grand). 2012 Jun 30;58 Suppl:OL1709-14.
Legionella pneumophila is a common cause of hospital and community-acquired pneumonia, being transmitted by inhalation of aqueous aerosols. Most legionellosis outbreaks are linked to contaminated hot water systems or cooling towers. The aim of this study was to determine the genetic diversity of (n= 55) environmental strains of L. pneumophila recovered from the hot water distribution systems of 16 establishments in seven Moroccan towns during the period 2009-2011. Thirteen chromosomal restriction patterns determined by Pulsed field gel electrophoresis were detected. The strains of L. pneumophila serogroup1 exhibited in 6/13 different PFGE patterns, while the strains of L. pneumophila serogroups 2-14 showed 7/13 PFGE patterns. The PFGE showed the existence of various patterns in Morocco, The pattern -XI- have tree similar profiles with the endemic L. pneumophila Paris's strain. This technique also allowed to conclude that the same pulsotype was found for many strains isolated from different establishments. Moreover, different pulsolypes were found for strains isolated from the same establishment. These results showed that PFGE analysis is a powerful tool to reveal the clonal nature and genetic differences among L. pneumophila strains.
嗜肺军团菌是医院获得性肺炎和社区获得性肺炎的常见病因，通过吸入含水气溶胶传播。大多数军团菌病暴发与受污染的热水系统或冷却塔有关。本研究的目的是确定2009年至2011年期间从摩洛哥七个城镇16个机构的热水分配系统中分离出的55株嗜肺军团菌环境菌株的遗传多样性。检测到通过脉冲场凝胶电泳确定的13种染色体限制性图谱。嗜肺军团菌血清群1的菌株呈现出6/13种不同的PFGE图谱，而嗜肺军团菌血清群2 - 14的菌株呈现出7/13种PFGE图谱。PFGE显示摩洛哥存在多种图谱，图谱 -XI- 与嗜肺军团菌巴黎地方菌株有三种相似的谱型。该技术还可以得出结论，从不同机构分离出的许多菌株具有相同的脉冲型。此外，从同一机构分离出的菌株发现了不同的脉冲型。这些结果表明，PFGE分析是揭示嗜肺军团菌菌株克隆性质和遗传差异的有力工具。
