Fares M, Armand M, Francois C, Maixent J M
INSERM U927, Faculté de Médecine, Université de Poitiers & CHU Miléterie, Poitiers, France.
Cell Mol Biol (Noisy-le-grand). 2012 Jun 30;58 Suppl:OL1715-9.
Polyunsaturated fatty acids (PUFA) supplementations modify cell lipid composition leading to a change in cell function. However, the effect of PUFA supplementations in renal model cell on the kidney epithelial cells membrane fatty acid profile is not known. Therefore, the purpose of this study was to determine the effects of PUFAs with different ω6/ω3 ratios supplementations in the kidney epithelial cells and the type of supplementation that can be used as cellular protection during kidney transplantation. For that, we used as model the LLCPK1 cell and determined their membrane fatty acid (FA) composition after supplementation with three different commercial food supplements. These supplements consist of S1: docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) with ω6/ω3 ratio = 0.1, S2: DHA, EPA, linoleic acid (LA) and γ-linoleic acid (GLA) with ω6/ω3 ratio = 2.5, or S3: α-linolenic acid (ALA) and LA with ω6/ω3 ratio near 1. Cells were incubated for 24 hr with 30 μM of ω3 fatty acids from each supplement. Fatty acid composition of control and experimental groups was analysed by gas chromatography after extraction of lipids and fatty acids methylation. The efficiency of cell PUFA supplementation was achieved by showing 2 to 4 fold increases in cell PUFA incorporation. Whatever the supplementation used, the cell saturated fatty acids (SFA) were decreased by 50% following the three supplementations used (p<0.001) as compared to control group. These decreases in SFA were compensated in part by increasing monounsaturated fatty acid levels. All these changes were observed with constant of cell ω6/ω3 ratio whatever the supplementations used. These data suggest that the supplements, with long chain polyunsaturated fatty acids or their precursors, lead to important regulation in the lipidome (desaturases and elongases) associated to preserved ω6/ω3 ratios. The fatty acids remodeling may represent an interesting new mechanism by which renal FA homoestasis could occurred.
多不饱和脂肪酸（PUFA）补充剂可改变细胞脂质组成，从而导致细胞功能发生变化。然而，PUFA补充剂对肾模型细胞中肾上皮细胞膜脂肪酸谱的影响尚不清楚。因此，本研究的目的是确定不同ω6/ω3比例的PUFA补充剂对肾上皮细胞的影响，以及在肾移植期间可作为细胞保护作用的补充剂类型。为此，我们以LLCPK1细胞为模型，在用三种不同的商业食品补充剂进行补充后，测定其膜脂肪酸（FA）组成。这些补充剂包括：S1：二十二碳六烯酸（DHA）和二十碳五烯酸（EPA），ω6/ω3比例 = 0.1；S2：DHA、EPA、亚油酸（LA）和γ-亚麻酸（GLA），ω6/ω3比例 = 2.5；或S3：α-亚麻酸（ALA）和LA，ω6/ω3比例接近1。将细胞与每种补充剂中的30 μM ω3脂肪酸孵育24小时。在脂质提取和脂肪酸甲基化后，通过气相色谱法分析对照组和实验组的脂肪酸组成。通过显示细胞PUFA掺入量增加2至4倍来实现细胞PUFA补充的效果。无论使用何种补充剂，与对照组相比，在使用三种补充剂后，细胞饱和脂肪酸（SFA）均降低了50%（p<0.001）。SFA的这些降低部分通过增加单不饱和脂肪酸水平得到补偿。无论使用何种补充剂，在细胞ω6/ω3比例恒定的情况下均观察到所有这些变化。这些数据表明，含有长链多不饱和脂肪酸或其前体的补充剂可导致与维持ω6/ω3比例相关的脂质组（去饱和酶和延长酶）发生重要调节。脂肪酸重塑可能代表了肾脂肪酸稳态发生的一种有趣的新机制。
