Aix-Marseille Universite, CRO2, 13385, Marseille, France; INSERM, UMR 911, 13385 Marseille, France.
Trends Biotechnol. 2012 Sep;30(9):466-74. doi: 10.1016/j.tibtech.2012.05.006. Epub 2012 Jul 2.
Mass spectrometry (MS)-based technology provides label-free localization of molecules in tissue samples. Drugs, proteins, lipids and metabolites can easily be monitored in their environment. Resolution can be achieved down to the cellular level (10-20 μm) for conventional matrix-assisted laser desorption/ionization (MALDI) imaging, or even to the subcellular level for more complex technologies such as secondary ionization mass spectrometry (SIMS) imaging. One question remains: are we going to be able to investigate functional relationships between drugs and proteins and compare with localized phenomena? This review describes the various spatial levels of investigation offered by mass spectrometry imaging (MSI), and the advantages and disadvantages compared with other labeling technologies.
基于质谱（MS）的技术提供了在组织样本中无标记分子定位的方法。可以轻松地在其环境中监测药物、蛋白质、脂质和代谢物。对于传统的基质辅助激光解吸/电离（MALDI）成像，可以实现分辨率达到细胞水平（10-20μm），对于更复杂的技术，如二次离子质谱（SIMS）成像，甚至可以达到亚细胞水平。目前仍有一个问题亟待解决：我们是否能够研究药物与蛋白质之间的功能关系，并将其与定位现象进行比较？本文综述了质谱成像（MSI）提供的各种空间研究水平，并与其他标记技术进行了比较。
