间质细胞中白细胞介素-6、白细胞介素-8 及其受体的表达与异位妊娠关系的研究
The expression of interleukin (IL)-6, IL-8, and their receptors in fallopian tubes with ectopic tubal gestation.
机构信息
Imperial College, London, United Kingdom.
出版信息
Fertil Steril. 2012 Oct;98(4):898-904. doi: 10.1016/j.fertnstert.2012.06.004. Epub 2012 Jul 2.
OBJECTIVE
To investigate the level of expression of the cytokines interleukin (IL) -8 and IL-6 and their receptors in fallopian tubes with tubal ectopic gestation.
DESIGN
Immunohistochemistry (IHC) was used to study the expression of IL-8, CXCR1, CXCR2, IL-6, and IL-6 receptor alpha in fallopian tubes with tubal ectopic gestation in comparison with normal fallopian tubes.
SETTING
Hospital.
PATIENT(S): We studied fallopian tubes from 50 patients with tubal ectopic gestation and normal fallopian tubes from 25 patients who had hysterectomy and bilateral salpingo-oopherectomy for uterine or ovarian lesions.
INTERVENTION(S): The expression of the chemokines/cytokines and their receptors was evaluated by IHC.
MAIN OUTCOME MEASURE(S): Level of expression and tissue distribution of the different antigens.
RESULT(S): IHC results showed that the expression levels of IL-6 and IL-8, as well as CXCR1, were significantly up-regulated, particularly near the implantation site in fallopian tubes with tubal gestation, while the expression levels of CXCR2 and IL-6Rα were not changed in comparison with normal fallopian tubes.
CONCLUSION(S): The results suggest a role for IL-6, IL-8, and CXCR1 in the pathophysiology of tubal ectopic gestation.
目的
探讨细胞因子白细胞介素（IL）-8 和 IL-6 及其受体在输卵管异位妊娠中的表达水平。
设计
采用免疫组织化学（IHC）方法研究输卵管异位妊娠与正常输卵管中 IL-8、CXCR1、CXCR2、IL-6 和 IL-6 受体α的表达。
地点
医院。
患者
我们研究了 50 例输卵管异位妊娠患者的输卵管和 25 例因子宫或卵巢病变行子宫双侧输卵管切除术的正常输卵管。
干预
通过 IHC 评估趋化因子/细胞因子及其受体的表达。
主要观察指标
不同抗原的表达水平和组织分布。
结果
免疫组化结果显示，IL-6 和 IL-8 以及 CXCR1 的表达水平明显上调，尤其是在输卵管妊娠的着床部位附近，而与正常输卵管相比，CXCR2 和 IL-6Rα 的表达水平没有变化。
结论
结果提示 IL-6、IL-8 和 CXCR1 在输卵管异位妊娠的病理生理学中起作用。
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