Ying Binbin, Chen Kan, Hu Jing, Man Cheng, Feng Ge, Zhang Bi, Zhu Songsong
The State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, China.
Br J Oral Maxillofac Surg. 2013 Apr;51(3):241-6. doi: 10.1016/j.bjoms.2012.05.014. Epub 2012 Jul 3.
Transforming growth factor-beta (TGF-β) plays an important part in the repair of cartilage in osteoarthritis. It has been hypothesised that intra-articular injection of TGF-β₁ promotes repair of cartilage and protects the subchondral bone from damage in osteoarthritic temporomandibular joints (TMJs). We made bilateral partial perforations of the disc to induce osteoarthritic joints in 36 rabbits. TGF-β₁ 20, 40, or 80 ng were injected into the right joint, and vehicle alone was injected into the left joint. Four additional animals were used as normal controls. Microcomputed tomography was used to quantify the three-dimensional microarchitecture of subchondral bone, followed by assessment of the proteoglycan content. All joints treated with TGF-β₁ were covered by a layer of well-organised fibrocartilage, and had increased proteoglycan content and normal microarchitectural properties, whereas the joint treated by vehicle alone had typical osteoarthritis-related degradation of cartilage and sclerosis of subchondral bone. These results suggested that TGF-β₁ is an effective way of treating osteoarthritis of the TMJ.
转化生长因子-β（TGF-β）在骨关节炎软骨修复中起重要作用。据推测，关节内注射TGF-β₁可促进骨关节炎颞下颌关节（TMJ）软骨修复并保护软骨下骨免受损伤。我们对36只兔进行双侧关节盘部分穿孔以诱导骨关节炎关节。将20、40或80 ng的TGF-β₁注入右侧关节，左侧关节仅注射赋形剂。另外4只动物用作正常对照。使用微型计算机断层扫描对软骨下骨的三维微结构进行量化，随后评估蛋白聚糖含量。所有用TGF-β₁治疗的关节均覆盖有一层组织良好的纤维软骨，蛋白聚糖含量增加且微结构特性正常，而仅用赋形剂治疗的关节则出现典型的与骨关节炎相关的软骨退变和软骨下骨硬化。这些结果表明，TGF-β₁是治疗TMJ骨关节炎的有效方法。
