Key Laboratory of Eco-chemical Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
Bioelectrochemistry. 2012 Dec;88:30-5. doi: 10.1016/j.bioelechem.2012.04.003. Epub 2012 Apr 20.
In this article, a simple and effective strategy for DNA immobilization on gold electrode surface is developed. The amine-modified oligonucleotide was firstly reacted with CS(2) and then in situ generated dithiocarbamate group functionalized probe DNA (DTC-DNA) was directly attached onto the gold surface by bidentate anchoring points. The DNA biosensor fabrication process was characterized by cyclic voltammetry and electrochemical impedance spectroscopy with the use of Fe(CN)(6)(3-/4-) as a redox indicator. The hybridization of DTC-DNA with complementary target DNA could be well distinguished with the use of Co(phen)(3)(3+) as an electrochemical indicator. The fabricated electrochemical DNA biosensor could achieve a detection limit of about 0.1nM toward complementary target DNA. Also, the current strategy is readily operated with less time consumed and lower cost compared with those commonly used strategies.
本文开发了一种将 DNA 固定在金电极表面的简单有效的策略。首先，将胺修饰的寡核苷酸与 CS(2)反应，然后原位生成二硫代氨基甲酸盐官能化探针 DNA(DTC-DNA)，通过双齿锚定点直接附着在金表面。通过使用 Fe(CN)(6)(3-/4-)作为氧化还原指示剂，用循环伏安法和电化学阻抗谱对 DNA 生物传感器的制备过程进行了表征。使用 Co(phen)(3)(3+)作为电化学指示剂，可以很好地区分 DTC-DNA 与互补目标 DNA 的杂交。所制备的电化学 DNA 生物传感器对互补目标 DNA 的检测限约为 0.1nM。此外，与常用策略相比，该策略操作简单，耗时少，成本低。
