Farooqui M Y, Cavazos R, Villarreal M I, Massa E
Department of Biological Sciences, University of Texas Pan American, Edinburg 78539.
Ecotoxicol Environ Saf. 1990 Oct;20(2):185-96. doi: 10.1016/0147-6513(90)90057-c.
The toxicity, uptake, tissue distribution, elimination, and covalent binding of 2-[14C]methyl-[2.3-14C]acrylonitrile (MeAN) in male Sprague-Dawley rats were investigated. Following an oral administration of 100 mg/Kg body weight (0.5 LD50, 8 microCi/Kg bw) the rats exhibited several signs of toxicity including ataxia, convulsions, mild diarrhea, salivation, lacrimation, and bladder urine retention. The treated animals excreted 43% of the 14C in the urine, 14% in the feces, and 2.5% in the expired air as 14CO2 in 10 days. Hydrogen cyanide was not detectable. Red blood cells retained significant amounts of radioactivity for more than 10 days after treatment. MeAN was extensively absorbed through the gastrointestinal tract and distributed in all the tissues of the rats. The major concentrations of the radioactivity were found with up to 25% of the administered dose in bone, liver, spleen, kidney, blood, and the gastrointestinal tract. This study indicates that MeAN is rapidly absorbed and distributed and the major route of excretion is urinary.
对雄性斯普拉格-道利大鼠体内2-[¹⁴C]甲基-[2,3-¹⁴C]丙烯腈（MeAN）的毒性、摄取、组织分布、消除及共价结合情况进行了研究。经口给予100mg/kg体重（0.5 LD₅₀，8μCi/kg体重）后，大鼠出现了多种中毒症状，包括共济失调、惊厥、轻度腹泻、流涎、流泪以及膀胱尿潴留。在10天内，经处理的动物尿液中排出了43%的¹⁴C，粪便中排出14%，呼出气体中以¹⁴CO₂形式排出2.5%。未检测到氰化氢。处理后，红细胞在10多天内仍保留大量放射性。MeAN通过胃肠道被大量吸收，并分布于大鼠的所有组织中。放射性主要集中在骨骼、肝脏、脾脏、肾脏、血液和胃肠道，剂量高达给药量的25%。本研究表明，MeAN吸收和分布迅速，主要排泄途径为尿液。
