Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 14220 Prague 4, Czech Republic.
Folia Microbiol (Praha). 2012 Sep;57(5):463-72. doi: 10.1007/s12223-012-0178-6. Epub 2012 Jul 5.
Plasmalogens are a group of lipids with potentially important, and not yet fully known, functions in organisms from bacteria to protozoans, invertebrates, and mammals. They can protect cells against the damaging effects of reactive oxygen species, protect other phospholipids or lipoprotein particles against oxidative stress, and have been implicated as signaling molecules and modulators of membrane dynamics. They have been found in many anaerobic bacterial species, and their biosynthetic pathways differ in aerobic and anaerobic organisms. The use of advanced techniques permits the identification of not only plasmalogen classes but also their positional isomers and often also individual molecular species. This paper describes direct analyses of plasmalogens from natural sources, frequently very unusual, using electrospray ionization mass spectrometry in combination with high-performance liquid chromatography and/or shotgun lipidomics.
磷脂酶是一组脂质，在从细菌到原生动物、无脊椎动物和哺乳动物等生物体中具有潜在重要但尚未完全了解的功能。它们可以保护细胞免受活性氧的破坏作用，保护其他磷脂或脂蛋白颗粒免受氧化应激，并且已经被牵连为信号分子和膜动力学调节剂。它们存在于许多厌氧细菌物种中，它们的生物合成途径在需氧和厌氧生物中不同。先进技术的应用不仅允许鉴定磷脂酶类，还可以鉴定其位置异构体，并且通常还可以鉴定单个分子种类。本文描述了使用电喷雾电离质谱结合高效液相色谱和/或 shotgun 脂质组学，直接分析来自天然来源的磷脂酶的方法，这些天然来源通常非常特殊。
