亲水作用液相色谱法：来自拜氏梭菌的缩醛磷脂的电喷雾串联质谱分析
Hydrophilic interaction liquid chromatography: ESI-MS/MS of plasmalogen phospholipids from Pectinatus bacterium.
作者信息
Rezanka Tomáš, Siristova Lucie, Matoulková Dagmar, Sigler Karel
机构信息
Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20, Prague, Czech Republic.
出版信息
Lipids. 2011 Aug;46(8):765-80. doi: 10.1007/s11745-011-3556-y. Epub 2011 Apr 11.
Liquid chromatography-electrospray tandem mass spectrometry (LC/ESI-MS/MS) was used to analyze phospholipids from three species of the anaerobic beer-spoilage bacterial genus Pectinatus. Analysis of total lipids by HILIC (Hydrophilic Interaction Liquid Chromatography) column succeeded in separating diacyl- and plasmalogen phospholipids. Plasmalogens were then analyzed by means of the ESI-MS/MS and more than 220 molecular species of four classes of plasmalogens (PlsCho (choline plasmalogen), PlsEtn (ethanolamine plasmalogen), PlsGro (glycerol plasmalogen), and PlsSer (serine plasmalogen)) were identified. Major molecular species were c-p19:0/15:0 PlsEtn and PlsSer, which accounted for more than 4% of the total lipids.
采用液相色谱 - 电喷雾串联质谱法（LC/ESI-MS/MS）分析了厌氧啤酒腐败细菌果胶杆菌属三种菌的磷脂。通过亲水相互作用液相色谱（HILIC）柱对总脂质进行分析，成功分离出二酰基磷脂和缩醛磷脂。然后借助电喷雾串联质谱法对缩醛磷脂进行分析，鉴定出四类缩醛磷脂（胆碱缩醛磷脂（PlsCho）、乙醇胺缩醛磷脂（PlsEtn）、甘油缩醛磷脂（PlsGro）和丝氨酸缩醛磷脂（PlsSer））的220多种分子种类。主要分子种类为c-p19:0/15:0 PlsEtn和PlsSer，占总脂质的4%以上。
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