Ławicki Sławomir, Będkowska Grażyna E, Gacuta-Szumarska Ewa, Knapp Paweł, Szmitkowski Maciej
Department of Biochemical Diagnostics, Medical University of Białystok, Poland.
Folia Histochem Cytobiol. 2012 Jul 4;50(2):213-9. doi: 10.5603/fhc.2012.0030.
In this study, we compared plasma levels and the diagnostic utility of hematopoietic growth factors (HGFs) with SCC-Ag in cervical cancer patients in relation to control groups and cervical intraepithelial neoplasia (CIN) patients and healthy subjects. Pretreatment plasma levels of HGFs (SCF, GM-CSF, G-CSF and M-CSF) were determined by the use of immunoenzyme assay (ELISA), and SCC-Ag by chemiluminescent microparticle immunoassay (CMIA). Significantly different concentrations of GM-CSF, G-CSF and M-CSF were observed in the group of patients with cervical cancer and CIN compared to the healthy controls. Significant differences in plasma levels of GM-CSF and M-CSF between cervical cancer and benign lesions patients were also found. The HGFs and SCC-Ag diagnostic specificities received high values. The diagnostic sensitivity and the predictive value of a positive and negative test result were higher for M-CSF than for antigen SCC in the cancer group. The M-CSF area under the ROC curve (AUC) was the largest from hematopoietic cytokines and SCC-Ag. These results suggest the potential utility of M-CSF as a good candidate for a marker of cervical cancer as well as benign lesions of this organ (CIN).
在本研究中，我们比较了宫颈癌患者与对照组、宫颈上皮内瘤变（CIN）患者及健康受试者的造血生长因子（HGFs）的血浆水平及其与鳞状细胞癌抗原（SCC-Ag）的诊断效用。通过免疫酶测定法（ELISA）测定HGFs（干细胞因子、粒细胞-巨噬细胞集落刺激因子、粒细胞集落刺激因子和巨噬细胞集落刺激因子）的预处理血浆水平，通过化学发光微粒免疫测定法（CMIA）测定SCC-Ag。与健康对照组相比，宫颈癌和CIN患者组中观察到粒细胞-巨噬细胞集落刺激因子、粒细胞集落刺激因子和巨噬细胞集落刺激因子的浓度有显著差异。在宫颈癌患者与良性病变患者之间，也发现粒细胞-巨噬细胞集落刺激因子和巨噬细胞集落刺激因子的血浆水平存在显著差异。HGFs和SCC-Ag的诊断特异性值较高。在癌症组中，巨噬细胞集落刺激因子的诊断敏感性以及阳性和阴性检测结果的预测值高于抗原SCC。巨噬细胞集落刺激因子的ROC曲线下面积（AUC）在造血细胞因子和SCC-Ag中是最大的。这些结果表明，巨噬细胞集落刺激因子作为宫颈癌以及该器官良性病变（CIN）标志物的良好候选者具有潜在效用。
