Yu Xia-Li, Jiang Xiao-Lin, Wang Tao, Sun Yu-Lan, Zhang Shuo, Li Chuan, Zhang Quan-Fu, Liang Mi-Fang, Bi Zhen-Qiang, Li De-Xin
National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China.
Bing Du Xue Bao. 2012 May;28(3):246-51.
Severe fever with thrombocytopenia syndrome bunyavirus is a newly emerging virus in China, enveloped with a tripartite, single-stranded RNA genome of negative polarity. The regulatory elements for viral transcription and replication, as well as encapsidation and packaging signals, are thought to be located within these noncoding regions (NCRs). The terminal nucleotides are genus specific and highly conserved. The function of the remaining nucleotides of the NCRs is still not well understood. In this study, we developed the plasmid-driven RNA polymerase I minireplicon system for SFTSV firstly, using reporter genes GFP and luciferase. The function of the noncoding regions of the three Bunyaviridae RNA segments (L, M, S) in transcription was analyzed. Reporter genes are successfully expressed in SFTSV minireplicon system. Our results suggest that the NCRs of SFTSV from all three segments contain the necessary signals to initiate transcription. Quantitative detection of the luciferase expression level shows that promoter activity in the three segments is different.
发热伴血小板减少综合征布尼亚病毒是中国新出现的一种病毒，具有包膜，其基因组为分节段的单链负链RNA。病毒转录、复制以及衣壳化和包装信号的调控元件被认为位于这些非编码区（NCR）内。末端核苷酸具有属特异性且高度保守。NCR其余核苷酸的功能仍未完全清楚。在本研究中，我们首先利用报告基因绿色荧光蛋白（GFP）和荧光素酶，开发了用于发热伴血小板减少综合征病毒（SFTSV）的质粒驱动RNA聚合酶I微型复制子系统。分析了布尼亚病毒科三个RNA节段（L、M、S）的非编码区在转录中的功能。报告基因在SFTSV微型复制子系统中成功表达。我们的结果表明，来自所有三个节段的SFTSV的NCR都包含启动转录所需的信号。荧光素酶表达水平的定量检测表明，三个节段中的启动子活性不同。
