Department of Microbiology and Immunology, Thomas Jefferson University, 233 S. 10th St, Philadelphia, PA 19107, USA.
Transgenic Res. 2013 Feb;22(1):179-85. doi: 10.1007/s11248-012-9629-8. Epub 2012 Jul 6.
The NOD.Cg-Prkdc ( scid ) Il2rg ( tm1Wjl )/SzJ mouse strain, commonly known as NSG (for NOD SCID Gamma) is severely immunodeficient and thus is an excellent recipient for xenografts, and in particular for engrafting human tumor cells and human hematopoietic stem cells. In the latter case, these cells give rise to many human hematopoetic lineages in their NSG hosts, resulting in recapitulation of many of the features of a human immune system. However, the immune system of these "humanized mice" (huMice) is not completely functional, in part because of a lack of expression of necessary human cytokines and HLA molecules by NSG host tissues. In order to facilitate the genetic modification of this strain in order to improve the huMouse model, we have created germline competent ES cells of this strain in which such modifications can be carried out.
NOD.Cg-Prkdc(scid)Il2rg(tm1Wjl)/SzJ 小鼠品系，通常被称为 NSG（意为 NOD SCID Gamma），是一种严重免疫缺陷的小鼠，因此是异种移植物的理想受体，特别是用于植入人肿瘤细胞和人造血干细胞。在后一种情况下，这些细胞在 NSG 宿主中产生许多人类造血谱系，从而再现了许多人类免疫系统的特征。然而，这些“人源化小鼠”（huMouse）的免疫系统并不完全功能正常，部分原因是 NSG 宿主组织缺乏表达必要的人类细胞因子和 HLA 分子。为了促进对这种品系的遗传修饰，以改进 huMouse 模型，我们已经创建了这种品系的种系嵌合 ES 细胞，可在这些细胞中进行此类修饰。
Zotero 插件